Phosphorylation of tick-borne encephalitis virus NS5 protein

被引:31
|
作者
Morozova, OV
Tsekhanovskaya, NA
Maksimova, TG
Bachvalova, VN
Matveeva, VA
Kit, YY
机构
[1] Siberian Division, Novosibirsk Inst. of Bioorg. Chem., Russian Academy of Sciences, Lavrentyev Prospect 8
关键词
tick-borne encephalitis virus; flavivirus NS5 protein; protein phosphorylation;
D O I
10.1016/S0168-1702(96)01433-5
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The largest tick-borne encephalitis virus (TBEV) non-structural protein NS5 (100 kDa) is believed to be involved in RNA replication. The protein is phosphorylated in infected cell extracts in the presence of [gamma-P-32]ATP, as shown by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis using monoclonal antibodies raised against TBEV NS5 protein. Radioactive labeling of NS5 in cellular extracts at an early stage post-infection is higher than at 24 h post-infection. Incubation of immunoprecipitates of NS5 protein with [gamma-P-32]ATP in the presence of Mg2+ resulted in the phosphorylation of TBEV NS5 protein and of immunoglobulins. Phosphoamino acid analysis demonstrated that NS5 contains phosphoserine, but not phosphothreonine, or phosphotyrosine. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:9 / 15
页数:7
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