tick-borne encephalitis virus;
flavivirus NS5 protein;
protein phosphorylation;
D O I:
10.1016/S0168-1702(96)01433-5
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
The largest tick-borne encephalitis virus (TBEV) non-structural protein NS5 (100 kDa) is believed to be involved in RNA replication. The protein is phosphorylated in infected cell extracts in the presence of [gamma-P-32]ATP, as shown by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis using monoclonal antibodies raised against TBEV NS5 protein. Radioactive labeling of NS5 in cellular extracts at an early stage post-infection is higher than at 24 h post-infection. Incubation of immunoprecipitates of NS5 protein with [gamma-P-32]ATP in the presence of Mg2+ resulted in the phosphorylation of TBEV NS5 protein and of immunoglobulins. Phosphoamino acid analysis demonstrated that NS5 contains phosphoserine, but not phosphothreonine, or phosphotyrosine. (C) 1997 Elsevier Science B.V.
机构:
Natl Inst Hlth Res, Res Hlth Protect Res Unit Emerging & Zoonot Infec, Liverpool, Merseyside, England
Publ Hlth England, Virol & Pathogenesis Grp, Natl Infect Serv, Porton Down, England
Publ Hlth England, Med Entomol & Zoonoses Ecol, Emergency Response Dept, Porton Down, EnglandNatl Inst Hlth Res, Res Hlth Protect Res Unit Emerging & Zoonot Infec, Liverpool, Merseyside, England
Holding, Maya
Dowall, Stuart D.
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Natl Inst Hlth Res, Res Hlth Protect Res Unit Emerging & Zoonot Infec, Liverpool, Merseyside, England
Publ Hlth England, Virol & Pathogenesis Grp, Natl Infect Serv, Porton Down, EnglandNatl Inst Hlth Res, Res Hlth Protect Res Unit Emerging & Zoonot Infec, Liverpool, Merseyside, England
Dowall, Stuart D.
Medlock, Jolyon M.
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h-index: 0
机构:
Natl Inst Hlth Res, Res Hlth Protect Res Unit Emerging & Zoonot Infec, Liverpool, Merseyside, England
Publ Hlth England, Med Entomol & Zoonoses Ecol, Emergency Response Dept, Porton Down, EnglandNatl Inst Hlth Res, Res Hlth Protect Res Unit Emerging & Zoonot Infec, Liverpool, Merseyside, England
Medlock, Jolyon M.
Carter, Daniel P.
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h-index: 0
机构:
Natl Inst Hlth Res, Res Hlth Protect Res Unit Emerging & Zoonot Infec, Liverpool, Merseyside, England
Publ Hlth England, Genom, Natl Infect Serv, Porton Down, EnglandNatl Inst Hlth Res, Res Hlth Protect Res Unit Emerging & Zoonot Infec, Liverpool, Merseyside, England
Carter, Daniel P.
Pullan, Steven T.
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Publ Hlth England, Genom, Natl Infect Serv, Porton Down, EnglandNatl Inst Hlth Res, Res Hlth Protect Res Unit Emerging & Zoonot Infec, Liverpool, Merseyside, England
Pullan, Steven T.
Lewis, James
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h-index: 0
机构:
Publ Hlth England, Geog Informat Syst, Emergency Response Dept, Porton Down, EnglandNatl Inst Hlth Res, Res Hlth Protect Res Unit Emerging & Zoonot Infec, Liverpool, Merseyside, England
Lewis, James
Vipond, Richard
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机构:
Natl Inst Hlth Res, Res Hlth Protect Res Unit Emerging & Zoonot Infec, Liverpool, Merseyside, England
Publ Hlth England, Virol & Pathogenesis Grp, Natl Infect Serv, Porton Down, EnglandNatl Inst Hlth Res, Res Hlth Protect Res Unit Emerging & Zoonot Infec, Liverpool, Merseyside, England
Vipond, Richard
Rocchi, Mara S.
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h-index: 0
机构:
Natl Inst Hlth Res, Res Hlth Protect Res Unit Emerging & Zoonot Infec, Liverpool, Merseyside, England
Moredun Res Inst, Virus Surveillance Unit, Edinburgh, Midlothian, ScotlandNatl Inst Hlth Res, Res Hlth Protect Res Unit Emerging & Zoonot Infec, Liverpool, Merseyside, England
Rocchi, Mara S.
Baylis, Matthew
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h-index: 0
机构:
Natl Inst Hlth Res, Res Hlth Protect Res Unit Emerging & Zoonot Infec, Liverpool, Merseyside, England
Univ Liverpool, Inst Infect & Global Hlth, Liverpool, Merseyside, EnglandNatl Inst Hlth Res, Res Hlth Protect Res Unit Emerging & Zoonot Infec, Liverpool, Merseyside, England
Baylis, Matthew
Hewson, Roger
论文数: 0引用数: 0
h-index: 0
机构:
Natl Inst Hlth Res, Res Hlth Protect Res Unit Emerging & Zoonot Infec, Liverpool, Merseyside, England
Publ Hlth England, Virol & Pathogenesis Grp, Natl Infect Serv, Porton Down, EnglandNatl Inst Hlth Res, Res Hlth Protect Res Unit Emerging & Zoonot Infec, Liverpool, Merseyside, England