Type 2 polarized immune response holds a major position in Epstein-Barr virus-related idiopathic thrombocytopenic purpura (EBV-ITP)

被引：19
作者：
Jin, C. -Q. [1 ]
Liu, F. [2 ]
Dong, H. -X. [1 ]
Zhang, J. [1 ]
Zhou, J. -W. [1 ]
Song, L. [2 ]
Xiao, H. [2 ]
Zheng, B. -Y. [2 ]
机构：
[1] Jining Med Coll, Analyt Sect Attached Hosp, Jining City, Shandong, Peoples R China
[2] Guang Dong Med Coll, Dept Microbiol & Immunol, Zhanjiang City, Guangdong, Peoples R China
来源：
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY | 2012年 / 34卷 / 02期
关键词：
Immunology; pediatrics; T cells; T-CELLS; INTERFERON-GAMMA; EXPRESSION; INTERLEUKIN-13; CYTOTOXICITY; LYMPHOCYTES; PLATELETS;
D O I：
10.1111/j.1751-553X.2011.01375.x
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Introduction: Dysfunctional cellular immunity is considered to be essential to the pathophysiology of EpsteinBarr virus-related idiopathic thrombocytopenic purpura (EBV-ITP). Cytokines in peripheral blood and the gene expression of transcription factors in T lymphocytes of patients with were investigated to correlate the polarization of T helper cell type 1 (Th1)/T helper cell type 2 (Th2) with the degree of thrombocytopenia. Methods: The expression of type 1 (T-bet) and type 2 (GATA-3) in T lymphocytes was detected by semiquantitative reverse-transcriptionpolymerase chain reaction, and plasma cytokine levels were measured by enzyme-linked immunoassay in 35 patients and 30 control subjects. Results: Th1/Th2 [(interleukin-2 + c-interferon)/(interleukin-10 + interleukin- 13)] cytokine ratios and transcription factor (T-bet/GATA-3) mRNA ratios were significantly decreased in patients with EBV-ITP, and the Th1/Th2 ratio was directly correlated with platelet counts. Conclusion: Our findings clearly show that type 2 polarization of the autoimmune response accounts for the expression of cytokines and transcription factors in EBV-ITP.
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收藏
页码：164 / 171
页数：8
相关论文
共 27 条
[1]   Expression of the Epstein-Barr virus-encoded Epstein-Barr virus nuclear antigen 1 in Hodgkin's lymphoma cells mediates up-regulation of CCL20 and the migration of regulatory T cells [J].
Baumforth, Karl R. N. ;
Birgersdotter, Anna ;
Reynolds, Gary M. ;
Wei, Wenbin ;
Kapatai, Georgia ;
Flavell, Joanne R. ;
Kalk, Emma ;
Piper, Karen ;
Lee, Steve ;
Machado, Lee ;
Hadley, Kerry ;
Sundblad, Anne ;
Sjoberg, Jan ;
Bjorkholm, Magnus ;
Porwit, Anna A. ;
Yap, Lee-Fah ;
Teo, Soohwang ;
Grundy, Richard G. ;
Young, Lawrence S. ;
Ernberg, Ingemar ;
Woodman, Ciaran B. J. ;
Murray, Paul G. .
AMERICAN JOURNAL OF PATHOLOGY, 2008, 173 (01) :195-204
[2]   Marked increased risk of Epstein-Barr virus-related complications with the addition of antithymocyte globulin to a nonmyeloablative conditioning prior to unrelated umbilical cord blood transplantation [J].
Brunstein, Claudio G. ;
Weisclorf, Daniel J. ;
DeFor, Todd ;
Barker, Juliet N. ;
Tolar, Jakub ;
van Burik, Jo-Anne H. ;
Wagner, John E. .
BLOOD, 2006, 108 (08) :2874-2880
[3]   Effects of dermatophagoides pteronyssinus allergen-specific immunotherapy on the serum interleukin-13 and pulmonary functions in asthmatic children [J].
Chen Zhuang-gui ;
Li Ming ;
Chen Yan-feng ;
Ji Jing-zhi ;
Li Ya-ting ;
Chen Wei ;
Chen Fen-hua ;
Chen Hong .
CHINESE MEDICAL JOURNAL, 2009, 122 (10) :1157-1161
[4]   Immunoregulatory effects of sinomenine on the T-bet/GATA-3 ratio and Th1/Th2 cytokine balance in the treatment of mesangial proliferative nephritis [J].
Cheng, Yue ;
Zhang, Jingbo ;
Hou, Weiping ;
Wang, Daihong ;
Li, Furong ;
Zhang, Yaoquan ;
Yuan, Fahuan .
INTERNATIONAL IMMUNOPHARMACOLOGY, 2009, 9 (7-8) :894-899
[5]   Medical progress: Immune thrombocytopenic purpura. [J].
Cines, DB ;
Blanchette, VS .
NEW ENGLAND JOURNAL OF MEDICINE, 2002, 346 (13) :995-1008
[6]   Interleukin-13 in the skin and interferon-γ in the liver are key players in immune protection in human schistosomiasis [J].
Dessein, A ;
Kouriba, B ;
Eboumbou, C ;
Dessein, H ;
Argiro, L ;
Marquet, S ;
Elwali, NEMA ;
Rodrigues, V ;
Li, YS ;
Doumbo, O ;
Chevillard, C .
IMMUNOLOGICAL REVIEWS, 2004, 201 :180-190
[7]  
Finotto Susetta, 2008, V94, P83, DOI 10.1159/000154869
[8]  
Gattoni A, 2006, Clin Ter, V157, P377
[9]   Th1 (CXCL10) and Th2 (CCL2) chemokine expression in patients with immune thrombocytopenia [J].
Gu, Dongsheng ;
Chen, Zhenping ;
Zhao, Haifeng ;
Du, Weiting ;
Xue, Feng ;
Ge, Jing ;
Sui, Tao ;
Wu, Hao ;
Liu, Bin ;
Lu, Shihong ;
Zhang, Lei ;
Yang, Renchi .
HUMAN IMMUNOLOGY, 2010, 71 (06) :586-591
[10]  
Jin Cheng-Qiang, 2009, Zhongguo Dang Dai Er Ke Za Zhi, V11, P367
123