Ovarian cancer marker of 11.7 kDa detected by proteomics is a serum amyloid A1

被引：88
作者：
Moshkovskii, SA
Serebryakova, MV
Kuteykin-Teplyakov, KB
Tikhonova, OV
Goufman, EI
Zgoda, VG
Taranets, IN
Makarov, OV
Archakov, AI
机构：
[1] Ctr Proteom, Inst Biomed Chem, Moscow 119121, Russia
[2] Russian State Med Univ, Moscow 117437, Russia
来源：
PROTEOMICS | 2005年 / 5卷 / 14期
关键词：
biomarker; ovarian cancer; SELDI; serum amyloid A;
D O I：
10.1002/pmic.200401205
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In this study, to reduce the number of major plasma components, we examined thermostable plasma fractions to search for a biomarker of ovarian cancer. An apparent cancer biomarker of 11.7 kDa was detected in these fractions using ProteinChip SELDI-TOF mass spectrometry System. This peak invariably appeared with another close peak of about 11.5 kDa, suggesting that it is a derivative of a larger mass molecule. Of 27 cancer plasma specimens, 15 (55.6%) demonstrated this peak pair, whereas only 2 of 34 controls specimens (5.8%) were shown to express it with low intensity. Using a method involving cysteine modification by 4-vinylpyridine (4-VP), 2DE and HPLC, these peaks were identified by mass spectrometry as serum amyloid A1 (11.68 kDa) and its N-terminal arginine-truncated form (11.52 kDa).
引用
收藏
页码：3790 / 3797
页数：8
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