High-Throughput Analysis from Complex Matrices: Acoustic Ejection Mass Spectrometry from Phase-Separated Fluid Samples

被引：13

作者：

Guo, Yuzhu ^{[1
]}

Forbush, Michael ^{[2
]}

Covey, Thomas R. ^{[1
]}

Ghislain, Lucien ^{[2
]}

Liu, Chang ^{[1
]}

机构：

[1] SCIEX Ltd, 71 Four Valley Dr, Concord, ON L4K 4V8, Canada

[2] Beckman Coulter Life Sci, 170 Rose Orchard Way, San Jose, CA 95134 USA

来源：

METABOLITES | 2021年 / 11卷 / 11期

关键词：

acoustic ejection mass spectrometry; high-throughput analysis; sample preparation; liquid-liquid extraction; FENTANYL; MICROEXTRACTION; INHIBITORS; INTERFACE;

D O I：

10.3390/metabo11110789

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Acoustic ejection mass spectrometry is a novel high-throughput analytical technology that delivers high reproducibility without carryover observed. It eliminates the chromatography step used to separate analytes from matrix components. Fully-automated liquid-liquid extraction is widely used for sample cleanup, especially in high-throughput applications. We introduce a workflow for direct AEMS analysis from phase-separated liquid samples and explore high-throughput analysis from complex matrices. We demonstrate the quantitative determination of fentanyl from urine using this two-phase AEMS approach, with a LOD lower than 1 ng/mL, quantitation precision of 15%, and accuracy better than & PLUSMN;10% over the range of evaluation (1-100 ng/mL). This workflow offers simplified sample preparation and higher analytical throughput for some bioanalytical applications, in comparison to an LC-MS based approach.

引用

页数：11

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