Genotyping of Brazilian Erysipelothrix spp. strains by amplified fragment length polymorphism

被引：4

作者：

Coutinho, Tania Alen ^{[1
]}

Imada, Yumiko ^{[2
]}

Santos Neves de Barcellos, David Emilio ^{[3
]}

de Oliveira, Sergio Jose ^{[4
]}

Moreno, Andrea Micke ^{[1
]}

机构：

[1] Univ Sao Paulo, Lab Sanidade Suina & Virol, FMVZ, BR-05508270 Sao Paulo, Brazil

[2] Natl Inst Anim Hlth, Dept Biol Prod, Tsukuba, Ibaraki 3050856, Japan

[3] Univ Fed Rio Grande Sul UFRGS, Setor Suinos, Fac Vet FAVET, BR-91540000 Porto Alegre, RS, Brazil

[4] Univ Luterana Brasil ULBRA, Lab Microbiol Clin & Alimentos, Fac Vet, BR-92425900 Sao Jose Dos Campos, Canoas, Brazil

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2011年 / 84卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

AFLP; Erysipelothrix spp; Genotyping; Serotyping; Swine; FIELD GEL-ELECTROPHORESIS; RAPID DETECTION; RHUSIOPATHIAE; PIGS; PCR; DNA; TONSILLARUM; AFLP; DIFFERENTIATION; SEROVARS;

D O I：

10.1016/j.mimet.2010.10.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

One hundred and fifty-one Erysipelothrix spp. isolates from diseased and carrier swine from Brazil were identified by PCR, submitted to serotyping and analyzed by amplified fragment length polymorphism with a single enzyme (AFLP). Reference strains from Australia and the United Kingdom were also examined. The 151 strains were classified into 18 different serotypes (1a, 1b, 2a, 2b, 4, 5, 6, 7, 8, 10, 11, 12, 15, 17, 19, 21, 24 and 25), being serotype 2b the most frequent (39.7%). By associating serotyping and PCR results, it was possible to identify 146 strains as E. rhusiopathiae and five strains as E. tonsillarum. Despite the fact that for this genus AFLP did not cluster all isolates according to serotype, origin, disease or isolation data, the execution of the technique was easy and fast, demonstrating high discriminatory power. The results produced by the AFLP analysis of Erysipelothrix spp. could also support its use as a discriminatory tool for E. rhusiopathiae and E. tonsillarum species. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：27 / 32

页数：6

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