Synthesis of a poly(Gd(III)-DOTA)-PNA conjugate as a potential MRI contrast agent via post-synthetic click chemistry functionalization

被引：5

作者：

Wang, Xiaoxiao ^{[1
]}

Milne, Mark ^{[1
]}

Martinez, Francisco ^{[2
]}

Scholl, Timothy J. ^{[2
]}

Hudson, Robert H. E. ^{[1
]}

机构：

[1] Univ Western Ontario, Dept Chem, 1151 Richmond St, London, ON N6A 5B7, Canada

[2] Univ Western Ontario, Robarts Res Inst, Dept Med Biophys, 1151 Richmond St, London, ON N6A 5B7, Canada

来源：

RSC ADVANCES | 2017年 / 7卷 / 71期

基金：

加拿大自然科学与工程研究理事会;

关键词：

PEPTIDE NUCLEIC-ACID; ALPHA-THIOSIALODENDRIMERS; MOLECULAR RECOGNITION; CELLULAR UPTAKE; BINDING; DENDRIMERS; DNA; PROTEIN; DESIGN; LECTIN;

D O I：

10.1039/c7ra09040d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Herein we present a method for the simultaneous, multiple conjugation of alkynyl-(Gd(III)-DOTA) moieties to a PNA oligomer possessing a domain of azide residues. This is achieved in a single Huisgen type coppercatalyzed azide alkyne cycloaddition (CuAAC) reaction after the oligomer assembly is completed. A triplex formed between the labelled PNA and riboadenylic acid, [(Gd(III)-DOTA)(4)-PNA](2):poly(rA), displayed higher relaxivities at any field strength compared to the single-stranded probe at the same concentration of Gd3+ ions as determined by nuclear magnetic resonance dispersion (NMRD) studies.

引用

页码：45222 / 45226

页数：5

共 7 条

[1] PNA Molecular Beacons Assembled by Post-Synthetic Click Chemistry Functionalization
Wang, Xiaoxiao
Hudson, Robert H. E.
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[2] Gd-DOTA Conjugate of RGD as a Potential Tumor-Targeting MRI Contrast Agent
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[3] Synthesis, characterization and relaxivity validations of Gd(III) complex of DOTA tetrahydrazide as MRI contrast agent
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[4] Lumazine Synthase Protein Nanoparticle-Gd(III)-DOTA Conjugate as a T1 contrast agent for high-field MRI
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[6] Synthesis of the Gd(III) complex with a tetrazole-armed macrocyclic ligand as a potential MRI contrast agent
Aime, S
Cravotto, G
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TETRAHEDRON LETTERS, 2002, 43 (05) : 783 - 786
[7] Synthesis and study on magnetic resonance imaging performance of Gd(III)-DTPA-bisfuran-2-carbohydrazide as a potential MRI contrast agent
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