A novel nacre protein N19 in the pearl oyster Pinctada fucata

被引：75
作者：
Yano, Masato
Nagai, Kouhei
Morimoto, Koichi
Miyamoto, Hiroshi [1 ]
机构：
[1] Kinki Univ, Sch Biol Oriented Sci & Technol, Dept Genet & Engn, Wakayama 6496493, Japan
[2] Japan Sci & Technol, Wakayam Prefecture Collaborat Reg Entities Adv T, Tokyo, Japan
[3] Kinki Univ, Sch Biol Oriented Sci & Technol, Dept Biotechnol Sci, Wakayama 6496493, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 362卷 / 01期
基金：
日本科学技术振兴机构;
关键词：
biomineralization; mollusc; pearl oyster; shell; MALDI-TOF/TOF;
D O I：
10.1016/j.bbrc.2007.07.172
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel 19 kDa protein, which was named N19, was isolated from the nacreous layer of the pearl oyster Pinetada fucata. N19 is one of predominant proteins found in the water-insoluble fraction of the nacreous layer. MALDI-TOF/TOF analysis indicated that the three trypsin-digested peptides (791.45, 824.42, and 1118.65 m/z) corresponded to the amino acid sequences predicted from a cDNA isolated from a mantle cDNA library of P. fucata. Northern blot analysis revealed that the N19 mRNA was a little more abundant in the pallial region than the edge region, in the mantle. In CaCO3 precipitation assay, the recombinant N19 protein inhibited the crystallization of CaCO3. These results indicate that N19 is localized in the nacre and plays a negative regulatory role in calcification in the pearl oyster. (C) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码：158 / 163
页数：6
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