Identification of miR-125a-5p as a tumor suppressor of renal cell carcinoma, regulating cellular proliferation, migration and apoptosis

被引：20
|
作者：
Chen, Duqun [1 ,2 ,3 ]
Li, Yifan [1 ,2 ,3 ]
Su, Zhengming [1 ,2 ,4 ]
Yu, Zuhu [1 ,2 ,3 ]
Yu, Wenshui [1 ,2 ,4 ]
Li, Yuchi [1 ,2 ,4 ]
Gui, Yaoting [2 ]
Yang, Shangqi [1 ,2 ]
Lai, Yongqing [1 ,2 ]
机构：
[1] Peking Univ, Dept Urol, Shenzhen Hosp, Shenzhen 518036, Guangdong, Peoples R China
[2] Peking Univ, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Shenzhen Hosp, Inst Urol Shenzhen PKU HKUST Med Ctr, Shenzhen 518036, Guangdong, Peoples R China
[3] Anhui Med Univ, Dept Urol, Hefei 230032, Anhui, Peoples R China
[4] Shantou Univ, Dept Urol, Coll Med, Shantou 515041, Guangdon, Peoples R China
来源：
MOLECULAR MEDICINE REPORTS | 2015年 / 11卷 / 02期
基金：
中国国家自然科学基金;
关键词：
miR-125a-5p; tumor suppressor; renal cell carcinoma; DOWN-REGULATION; GASTRIC-CANCER; MICRORNA; PERTUZUMAB; SERUM;
D O I：
10.3892/mmr.2014.2848
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
miR-125a-5p has been previously described as a tumor suppressor in numerous malignancies, however the expression and function of miR-125a-5p in renal cell carcinoma (RCC) remains to be elucidated. In the present study, to explore the potential role of miR-125a-5p in RCC, quantitative polymerase chain reaction was used to determine the expression levels of miR-125a-5p in renal cancer tissues. The influence of miR-125a-5p on cell proliferation, migration and apoptosis was also determined, using an MTT assay, a wound scratch assay and flow cytometry, respectively. The expression of miR-125a-5p was shown to be decreased in RCC and the restoration of miR-125a-5p by synthetic mimics was shown to suppress cell proliferation and migration, and induce apoptosis. The present results indicate that miR-125a-5p may function as a tumor suppressor in RCC. The present study is, to the best of our knowledge, the first to demonstrate the downregulation of miR-125a-5p in RCC, and to show the role it has in affecting cellular proliferation, migration and apoptosis. Further research is needed to define the target genes of miR-125a-5p and explore the potential of miR-125a-5p as a diagnostic or a prognostic biomarker for RCC.
引用
收藏
页码：1278 / 1283
页数：6
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