Competitive binding of Chlorin p6 and Dansyl-L-Proline to Sudlow's site II of human serum albumin

被引：17

作者：

Patel, Sunita ^{[1
]}

Sharma, Kaushal Kishor ^{[2
]}

Datta, Anindya ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Chem, Mumbai 400076, Maharashtra, India

[2] Indian Inst Sci, Mol Biophys Unit, Bangalore 560012, Karnataka, India

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2015年 / 138卷

关键词：

Chlorin; Specific binding; Sudlow's site; Stopped-flow; Time resolved fluorescence; VIVO PHOTOSENSITIZING EFFICACY; PHOTODYNAMIC THERAPY; PH; STATE; DERIVATIVES; CURCUMIN; AFFINITY; RECEPTOR; MODEL;

D O I：

10.1016/j.saa.2014.10.015

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

The binding of chlorin p(6), a model photosensitizer for photodynamic therapy (PDT), to the Sudlow's site II of Human Serum Albumin (HSA) has been monitored by different spectroscopic methods. Displacement of Dansyl-L-Proline (DP) from its conjugate with HSA is manifested in the spectral shift and decrease in its fluorescence intensity as well as the emergence of component with lifetime of 2-3 ns, which is characteristic of free DP. As DP is known to bind specifically to the Sudlow's site II of human serum albumin, its displacement by chlorin p(6) indicates the residence of the photosensitizer in the same site, in addition to Sudlow's site I. The binding constants for Sudlow's site II, determined by the stopped-flow technique, are found to be two orders of magnitude smaller than that for Sudlow's site I. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：925 / 931

页数：7

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