Aggressiveness and diversity of Phytophthora capsici on vegetable crops in Georgia

被引:53
作者
Yin, J. [1 ]
Jackson, K. L. [1 ]
Candole, B. L. [2 ]
Csinos, A. S. [1 ]
Langston, D. B. [1 ]
Ji, P. [1 ]
机构
[1] Univ Georgia, Coastal Plain Expt Stn, Dept Plant Pathol, Tifton, GA 31794 USA
[2] Univ Georgia, Dept Plant Pathol, Athens, GA 30602 USA
关键词
Genetic diversity; mating type; Phytophthora blight; pathotype; RAPD; GENETIC-VARIATION; CUCURBIT FIELDS; NORTH-CAROLINA; PEPPER FIELDS; SP-NOV; BLIGHT; RESISTANCE; VIRULENCE; MEFENOXAM; PUMPKIN;
D O I
10.1111/j.1744-7348.2012.00532.x
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Phytophthora blight induced by Phytophthora capsicicauses significant yield loss in a number of vegetable crops. It is imperative to understand the diversity and aggressiveness of the pathogen to design more efficient disease management programs. A collection of P. capsici strains isolated from different vegetable crops in Georgia, USA, were characterised in this study. Of the 49 isolates tested, 24 were A1 and 25 were A2 mating type, respectively, with both mating types found in the same fields. Variability of the isolates was assessed in terms of their aggressiveness on six pepper genotypes. The isolates differed in their aggressiveness on different pepper cultivars with 10 pathotypes identified. No correlation between aggressiveness of the isolates and their host origin or geographical location of isolation was observed. Randomly amplified polymorphic DNA (RAPD) analysis was used to evaluate genetic variability among P. capsici populations. RAPD analysis using 15 random primers resulted in 133 reproducible bands and cluster analysis separated the isolates into 5 groups. Analysis of molecular variance showed that there was moderate genetic differentiation associated with host origin and geographical location of the isolates. No correlation was found between RAPD groups and pathotypes or mating types. These results indicate that P. capsici populations infecting vegetable crops in Georgia were genetically diverse, which should be taken into account in developing resistant cultivars or other disease management programmes.
引用
收藏
页码:191 / 200
页数:10
相关论文
共 39 条
[1]  
[Anonymous], 1978, EVOLUTION GENETICS P, DOI DOI 10.2307/1224510
[2]  
[Anonymous], 2000, ARLEQUIN SOFTWARE PO
[3]   Morphological distinctions between Phytophthora capsici and P-tropicalis sp nov. [J].
Aragaki, M ;
Uchida, JY .
MYCOLOGIA, 2001, 93 (01) :137-145
[4]   Genetic and morphological diversity of temperate and tropical isolates of Phytophthora capsici [J].
Bowers, J. H. ;
Martin, F. N. ;
Tooley, P. W. ;
Luz, E. D. M. N. .
PHYTOPATHOLOGY, 2007, 97 (04) :492-503
[5]   RELATIONSHIP BETWEEN INOCULUM LEVEL OF PHYTOPHTHORA-CAPSICI AND MORTALITY OF PEPPER [J].
BOWERS, JH ;
MITCHELL, DJ .
PHYTOPATHOLOGY, 1991, 81 (02) :178-184
[6]   Fitness of isolates of Phytophthora capsici resistant to mefenoxam from squash and pepper fields in North Carolina [J].
Cafe-Filho, Adalberto C. ;
Ristaino, Jean Beagle .
PLANT DISEASE, 2008, 92 (10) :1439-1443
[7]   Population Structure and Resistance to Mefenoxam of Phytophthora capsici in New York State [J].
Dunn, A. R. ;
Milgroom, M. G. ;
Meitz, J. C. ;
McLeod, A. ;
Fry, W. E. ;
McGrath, M. T. ;
Dillard, H. R. ;
Smart, C. D. .
PLANT DISEASE, 2010, 94 (12) :1461-1468
[8]  
Erwin CE., 1996, Phytophthora Diseases Worldwide
[9]   Resistance of Pepper to Phytophthora Crown, Root, and Fruit Rot Is Affected by Isolate Virulence [J].
Foster, J. M. ;
Hausbeck, M. K. .
PLANT DISEASE, 2010, 94 (01) :24-30
[10]  
Gallegly ME., 2008, PHYTOPHTHORA IDENTIF