Establishment and characterization of a new cell line of Chilo suppressalis Walker (Lepidoptera: Pyralididae)

被引：5

作者：

Liu, Guangfu ^{[1
,2
]}

Xu, Yipeng ^{[1
]}

Yu, Xiaoping ^{[1
]}

机构：

[1] China Jiliang Univ, Zhejiang Prov Key Lab Biometrol & Inspect & Quara, Coll Life Sci, Hangzhou 310018, Zhejiang, Peoples R China

[2] Anhui Agr Univ, Hefei 230036, Peoples R China

来源：

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL | 2015年 / 51卷 / 03期

基金：

国家高技术研究发展计划(863计划);

关键词：

Chilo suppressalis Walker; Cell line; DAF-PCR; LARVAL FAT-BODIES; SPODOPTERA-EXIGUA; NOCTUIDAE; ARMYWORM; VIRUS;

D O I：

10.1007/s11626-014-9831-5

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A new cell line, designated as ZJBIQ-Chsu-I, was initiated from the fat body of larval Chilo suppressalis (Walker) (Lepidoptera: Pyralididae) in TNM-FH insect medium containing 15% fetal bovine serum. The polygonal cells (65.6%) were predominant among various cell types, and the diameter range was from 12.63 to 22.50 mu m. The cell line showed a typical lepidopteran chromosome pattern ranging from 108 to 136 chromosomes in the majority of the cells. The population doubling time (PDT) of the cell line at the 15th passage was 62 h. This cell line was found to be susceptible to Spodoptera exigua nuclear polyhedrosis virus (SeNPV). By the DNA amplification fingerprinting polymerase chain reaction (DAF-PCR) technique, it was confirmed that cell line ZJBIQ-Chsu-I really originated from C. suppressalis.

引用

页码：218 / 221

页数：4

共 23 条

[1]

Cai Wanzhi, 1994, Entomotaxonomia, V16, P4

[2]

EIDE PE, 1975, IN VITRO CELL DEV B, V11, P395

[3] Establishment and characterization of insect cell lines from 10 lepidopteran species [J].