Monitoring the conjugal transfer of plasmid RP4 in activated sludge and in situ identification of the transconjugants

被引：48

作者：

Geisenberger, O

Ammendola, A

Christensen, BB

Molin, S

Schleifer, KH

Eberl, L

机构：

[1] Tech Univ Munich, Lehrstuhl Mikrobiol, D-80290 Munich, Germany

[2] Tech Univ Denmark, Dept Microbiol, DK-2800 Lyngby, Copenhagen, Denmark

来源：

FEMS MICROBIOLOGY LETTERS | 1999年 / 174卷 / 01期

关键词：

plasmid transfer; green fluorescent protein; activated sludge; plasmid RP4;

D O I：

10.1016/S0378-1097(99)00114-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A GFPmut3b-tagged derivative of broad host-range plasmid RP4 was used to monitor the conjugative transfer of the plasmid from a Pseudomonas putida donor strain to indigenous bacteria in activated sludge. Transfer frequencies were determined to be in the range of 4x10(-6) to 1x10(-5) transconjugants per recipient. In situ hybridisation with fluorescently labeled, rRNA-targeted oligonucleotides was used to phylogenetically affiliate the bacteria that had received the plasmid. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：9 / 17

页数：9

共 19 条

[1] In situ visualization of high genetic diversity in a natural microbial community [J].