Hepatic DNA Methylation in Response to Early Stimulation of Microbiota with Lactobacillus Synbiotics in Broiler Chickens

被引：23
作者：
Dunislawska, Aleksandra [1 ]
Slawinska, Anna [1 ]
Siwek, Maria [1 ]
机构：
[1] UTP Univ Sci & Technol, Dept Anim Biotechnol & Genet, PL-85796 Bydgoszcz, Poland
来源：
GENES | 2020年 / 11卷 / 05期
关键词：
gut-liver axis; gene silencing; prebiotic; probiotic; ORPHAN NUCLEAR RECEPTOR; GENE; HYPERMETHYLATION; PREBIOTICS; LIVER; NOR1;
D O I：
10.3390/genes11050579
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
DNA methylation inhibits DNA transcription by the addition of methyl residues to cysteine within the CpG islands of gene promoters. The process of DNA methylation can be modulated by environmental factors such as intestinal microbiota. In poultry, the composition of the intestinal microbiota can be stimulated by in ovo delivery of synbiotics. The present study aims to determine the effect ofLactobacillussynbiotics delivered in ovo on the level of hepatic DNA methylation in broiler chickens. In ovo stimulation was performed on day 12 of egg incubation. Bioactive compounds delivered in ovo included (S1)-Lactobacillus salivariuswith GOS and (S2)-Lactobacillus plantarumwith RFO. Samples were collected from six individuals from each group on day 42 post-hatching. DNA methylation of five genes selected on the basis of the transcriptome data were analyzed using the qMSP method. Significant changes were observed in DNA methylation of genes in liver includingANGPTL4andNR4A3, after S2 delivery. The obtained results confirm that the downregulation of metabolic gene expression in the liver mediated by in ovo stimulation had epigenetic characteristics.
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页数：9
相关论文
共 36 条
[1]  
[Anonymous], 1982, Molecular Cloning
[2]   The microbiota programs DNA methylation to control intestinal homeostasis and inflammation [J].
Ansari, Ihab ;
Raddatz, Guenter ;
Gutekunst, Julian ;
Ridnik, Meshi ;
Cohen, Daphne ;
Abu-Remaileh, Monther ;
Tuganbaev, Timur ;
Shapiro, Hagit ;
Pikarsky, Eli ;
Elinav, Eran ;
Lyko, Frank ;
Bergman, Yehudit .
NATURE MICROBIOLOGY, 2020, 5 (04) :610-+
[3]   Decreased Fat Storage by Lactobacillus Paracasei Is Associated with Increased Levels of Angiopoietin-Like 4 Protein (ANGPTL4) [J].
Aronsson, Linda ;
Huang, Ying ;
Parini, Paolo ;
Korach-Andre, Marion ;
Hakansson, Janet ;
Gustafsson, Jan-Ake ;
Pettersson, Sven ;
Arulampalam, Velmurugesan ;
Rafter, Joseph .
PLOS ONE, 2010, 5 (09) :1-7
[4]   Influence of different prebiotics and mode of their administration on broiler chicken performance [J].
Bednarczyk, M. ;
Stadnicka, K. ;
Kozlowska, I. ;
Abiuso, C. ;
Tavaniello, S. ;
Dankowiakowska, A. ;
Slawinska, A. ;
Maiorano, G. .
ANIMAL, 2016, 10 (08) :1271-1279
[5]   Transcriptome modulation by in ovo delivered Lactobacillus synbiotics in a range of chicken tissues [J].
Dunislawska, A. ;
Slawinska, A. ;
Bednarczyk, M. ;
Siwek, M. .
GENE, 2019, 698 :27-33
[6]  
DUNISLAWSKA A, 2020, ANIMALS BASEL, V0010
[7]   Synbiotics for Broiler Chickens-In Vitro Design and Evaluation of the Influence on Host and Selected Microbiota Populations following In Ovo Delivery [J].
Dunislawska, Aleksandra ;
Slawinska, Anna ;
Stadnicka, Katarzyna ;
Bednarczyk, Marek ;
Gulewicz, Piotr ;
Jozefiak, Damian ;
Siwek, Maria .
PLOS ONE, 2017, 12 (01)
[8]   Quantitative multiplex methylation-specific PCR assay for the detection of promoter hypermethylation in multiple genes in breast cancer [J].
Fackler, MJ ;
McVeigh, M ;
Mehrotra, J ;
Blum, MA ;
Lange, J ;
Lapides, A ;
Garrett, E ;
Argani, P ;
Sukumar, S .
CANCER RESEARCH, 2004, 64 (13) :4442-4452
[9]   DNA Methylation Analysis of the Gene CDKN2B in Gallus gallus (Chicken) [J].
Gryzinska, Magdalena ;
Andraszek, Katarzyna ;
Jocek, Grzegorz .
FOLIA BIOLOGICA-KRAKOW, 2013, 61 (3-4) :165-171
[10]   Analysis of Age-Related Global DNA Methylation in Chicken [J].
Gryzinska, Magdalena ;
Blaszczak, Ewa ;
Strachecka, Aneta ;
Jezewska-Witkowska, Grazyna .
BIOCHEMICAL GENETICS, 2013, 51 (7-8) :554-563
1234