Detection and spatial distribution of IL-2 receptors on mouse T-lymphocytes by immunogold-labeled ligands

To identify the plasma membrane (PM) structures implicated in T-cell activation, we studied the distribution of interleukin-2 receptors (IL-2R) and the surface topography of lymphocytes by affinity labeling in electron microscopy (EM), In particular, we analyzed the distribution of the IL-2R alpha-chain on CTLL-2 cells (a murine cytotoxic T-cell lymphoma line), Some of our experiments were extended to the functionally and morphologically distinct cell line EL4 (a murine helper T-cell lymphoma line), As affinity ligands we used a rat monoclonal antibody (clone 7D4) reactive with the murine a-chain of IL-2R and recombinant mouse IL-2 (rIL-2). The distribution of IL-2R was visualized on the cell surface by ligands coupled to colloidal gold particles of different sizes, Unfixed cells were labeled with gold probes and attached to concanavalin A (ConA)-pretreated coverslips. Subsequently, the cells were prepared for EM. Examination of ultrathin sections and large surface replicas revealed a high degree of variability in cell morphology and in the density of the randomly distributed gold-labeled ligands among CTLL cells, According to their typical appearance, lymphocytes with strong receptor expression can be easily identified within the cell population, In contrast, the label on many mitogen-activated EL4 cells showed a cap-like polar distribution, The results suggest the existence of diverse distribution patterns of IL-2R on CTLL and EL4 cells, These differences are believed to reflect the different physiological roles played by T-cell subsets in the immune system.