Differentiation between Staphylococcus aureus and Staphylococcus epidermidis strains using Raman spectroscopy

被引：18

作者：

Rebrosova, Katarina ^{[1
,2
]}

Siler, Martin ^{[3
]}

Samek, Ota ^{[3
]}

Ruzicka, Filip ^{[1
,2
]}

Bernatova, Silvie ^{[3
]}

Jezek, Jan ^{[3
]}

Zemanek, Pavel ^{[3
]}

Hola, Veronika ^{[1
,2
]}

机构：

[1] Fac Med, Dept Microbiol, Pekarska 53, Brno 65691, Czech Republic

[2] St Annes Fac Hosp, Pekarska 53, Brno 65691, Czech Republic

[3] ASCR, Inst Sci Instruments, Kralovopolska 147, Brno 61264, Czech Republic

来源：

FUTURE MICROBIOLOGY | 2017年 / 12卷 / 10期

关键词：

Raman spectroscopy; rapid diagnostics; Staphylococcus epidermidis; Staphyococcus aureus; COAGULASE-NEGATIVE STAPHYLOCOCCI; BIOFILM FORMATION; IDENTIFICATION; BACTERIA; PATHOGENS; CELLS; MICROSPECTROSCOPY; SPECTRA;

D O I：

10.2217/fmb-2016-0224

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Aim: Raman spectroscopy is an analytical method with a broad range of applications across multiple scientific fields. We report on a possibility to differentiate between two important Gram-positive species commonly found in clinical material - Staphylococcus aureus and Staphylococcus epidermidis - using this rapid noninvasive technique. Materials & methods: For this, we tested 87 strains, 41 of S. aureus and 46 of S. epidermidis, directly from colonies grown on a Mueller-Hinton agar plate using Raman spectroscopy. Discussion & conclusion: The method paves a way for separation of these two species even on high number of samples and therefore, it can be potentially used in clinical diagnostics.

引用

页码：881 / 890

页数：10

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