Efficient production of extracellular alkaline protease in Bacillus amyloliquefaciens by host strain construction

被引:16
作者
Chen, Weijie [1 ]
Li, Lu [2 ]
Ye, Changwen [3 ]
Zhao, Ziyue [1 ]
Huang, Kuo [3 ]
Zou, Dian [1 ]
Wei, Xuetuan [1 ]
机构
[1] Huazhong Agr Univ, Coll Food Sci & Technol, Key Lab Environm Correlat Dietol, Minist Educ, Wuhan 430070, Peoples R China
[2] Guangdong Acad Agr Sci, Sericultural & Argi Food Res Inst, Key Lab Funct Foods, Guangdong Key Lab Agr Prod Proc,Minist Agr & Rural, Guangzhou 510610, Peoples R China
[3] China Natl Tobacco Corp, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Peoples R China
关键词
B; amyloliquefaciens; Gene knock-out; Host modification; Alkaline protease; EXPRESSION; SUBTILIS; CHITOSANASE; DEFICIENT; CLONING; GENE;
D O I
10.1016/j.lwt.2022.113620
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Bacillus amyloliquefaciens is an important industrial microorganism, which can be applied as a protein expression host. However, the extracellular protein expression ability of B. amyloliquefaciens is still low. Hence, it is necessary to develop a B. amyloliquefaciens host with a high extracellular protein expression capacity. In this study, B. amyloliquefaciens HZ-12 was the original strain, then seven important extracellular protease genes (epr, nprE, aprE-a, mpr, pbpF, vpr, ykct1), one important intracellular protease gene (aprX) and two redundant proteins genes (htrB, hag) were deleted in HZ-12, resulting in B. amyloliquefaciens BAX-10. In order to evaluate the extracellular protein expression ability of BAX-10, the alkaline protease gene aprE from Bacillus subtilis D7 was expressed in BAX-10. After fermentation, the alkaline protease activity of BAX-10/aprE reached 666.82 U/mL, which was 57% higher than that of the control strain HZ/aprE. Moreover, the deletion of the ten genes had no negative effect on the growth of B. amyloliquefaciens. In summary, B. amyloliquefaciens BAX-10 could be used as a potential platform host for expression of target extracellular proteins.
引用
收藏
页数:7
相关论文
共 33 条
[1]   Recent advances in microbial glutaminase production and applications-a concise review [J].
Amobonye, Ayodeji ;
Singh, Suren ;
Pillai, Santhosh .
CRITICAL REVIEWS IN BIOTECHNOLOGY, 2019, 39 (07) :944-963
[2]   Application of a new xylanase activity from Bacillus amyloliquefaciens XR44A in brewer's spent grain saccharification [J].
Amore, Antonella ;
Parameswaran, Binod ;
Kumar, Ramesh ;
Birolo, Leila ;
Vinciguerra, Roberto ;
Marcolongo, Loredana ;
Ionata, Elena ;
La Cara, Francesco ;
Pandey, Ashok ;
Faraco, Vincenza .
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 2015, 90 (03) :573-581
[3]   Impact of microbial proteases on biotechnological industries [J].
Banerjee, Goutam ;
Ray, Arun Kumar .
BIOTECHNOLOGY AND GENETIC ENGINEERING REVIEWS, VOL 33, ISSUE 2, 2017, 33 (02) :119-143
[4]   New Class of Chitosanase from Bacillus amyloliquefaciens for the Generation of Chitooligosaccharides [J].
Bhuvanachandra, Bhoopal ;
Sivaramakrishna, Dokku ;
Alim, Sk ;
Preethiba, Gopi ;
Rambabu, Samudrala ;
Swamy, Musti J. ;
Podile, Appa Rao .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2021, 69 (01) :78-87
[5]   An overview of Bacillus proteases: from production to application [J].
Contesini, Fabiano Jares ;
de Melo, Ricardo Rodrigues ;
Sato, Helia Harumi .
CRITICAL REVIEWS IN BIOTECHNOLOGY, 2018, 38 (03) :321-334
[6]   Cloning and expression of Staphylococcus simulans lysostaphin enzyme gene in Bacillus subtilis WB600 [J].
Far, Babak Elyasi ;
Ragheb, Mehran ;
Rahbar, Reza ;
Mafakher, Ladan ;
Nojookambari, Neda Yousefi ;
Achinas, Spyridon ;
Yazdansetad, Sajjad .
AIMS MICROBIOLOGY, 2021, 7 (03) :271-283
[7]   Gene cloning and expression of the L-asparaginase from Bacillus cereus BDRD-ST26 in Bacillus subtilis WB600 [J].
Feng, Yue ;
Liu, Song ;
Jiao, Yun ;
Wang, Yunlong ;
Wang, Miao ;
Du, Guocheng .
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2019, 127 (04) :418-424
[8]   A systematic reconsideration on proteases [J].
Gurumallesh, Poorani ;
Alagu, Kamalini ;
Ramakrishnan, Baskar ;
Muthusamy, Shanmugaprakash .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2019, 128 :254-267
[9]   Response-surface methodology for the production and the purification of a new H2O2-tolerant alkaline protease from Bacillus invictae AH1 strain [J].
Hammami, Aural ;
Bayoudh, Ahmed ;
Hadrich, Bilel ;
Abdelhedi, Ola ;
Jridi, Mourad ;
Nasri, Moncef .
BIOTECHNOLOGY PROGRESS, 2020, 36 (03)
[10]  
Huang W. J, 2012, AFR J BIOTECHNOL, V11