Protective Effects of Peroxisome Proliferator-Activated Receptor-α Agonist, Wy14643, on Hypoxia/Reoxygenation Injury in Primary Rat Hepatocytes

被引：6
作者：
Chen, Ke [1 ]
Li, Yuan-Hai [1 ]
Xu, Si-Qi [1 ]
Hu, Sheng-Hong [1 ]
Zhang, Lei [2 ]
机构：
[1] Anhui Med Univ, Affiliated Hosp 1, Dept Anesthesiol, Hefei 230022, Peoples R China
[2] Anhui Med Univ, Dept Pharmacol, Hefei 230022, Peoples R China
来源：
PPAR RESEARCH | 2012年 / 2012卷
关键词：
ISCHEMIA-REPERFUSION INJURY; GENE-EXPRESSION; HYPOXIA; GLUTATHIONE; CELLS; ISCHEMIA/REPERFUSION; INFLAMMATION; MECHANISM; SURVIVAL; HEART;
D O I：
10.1155/2012/547980
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
This study investigates the effects and possible mechanism of an agonist of PPAR alpha, Wy14643, on primary hepatocytes subjected to H/R injury in rats. H/R induced a significant increase ALT, AST, MDA in the culture medium and ROS in the hepatocytes. These effects were reversed by pretreatment with Wy14643 in the dose-dependent manner. The activity of SOD and the level of GSH in the hepatocytes were decreased after H/R, which were increased by Wy14643 pretreatment. Moreover, the mRNA expressions of PPAR alpha significantly increased in H/R+Wy14643 groups when compared with that in H/R group. A PPAR alpha agonist, Wy14643, exerts significant protective effect against H/R injury in primary hepatocytes via PPAR alpha activation and attenuating oxidative stress.
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页数：8
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