Nanocapsule of cationic liposomes obtained using "in situ" acrylic acid polymerization: Stability, surface charge and biocompatibility

被引：13

作者：

Scarioti, Giovana Danieli ^{[2
]}

Lubambo, Adriana ^{[1
]}

Feitosa, Judith P. A. ^{[3
]}

Sierakowski, Maria Rita ^{[1
]}

Bresolin, Tania M. B. ^{[2
]}

de Freitas, Rilton Alves ^{[1
,2
]}

机构：

[1] Univ Fed Parana UFPR, Dept Quim, BioPol, BR-81531990 Curitiba, Parana, Brazil

[2] Univ Vale Itajai UNIVALI, Nucleo Investigacoes Quim Farmaceut NIQFAR, BR-88302202 Itajai, Santa Catarina, Brazil

[3] Univ Fed Ceara, Dept Quim Organ & Inorgan, BR-60455760 Fortaleza, Ceara, Brazil

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2011年 / 87卷 / 02期

关键词：

Cationic liposomes; Nanocapsules; Long-circulating liposomes; Highly interactive liposomes; Cytotoxicity; GENE-TRANSFER; TOXICITY; LIPIDS; DELIVERY; MECHANISM; VESICLES; PROGRESS; VECTORS; AGENTS;

D O I：

10.1016/j.colsurfb.2011.05.027

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this work, didecyldimethylammonium bromide (DDAB) and 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine (DOPE) (2.5:1) were used to prepare liposomes coated with polyacrylic acid (PAA) using "in situ" polymerization with 2.5, 5 and 25 mM of acrylic acid (AA). The PAA concentrations were chosen to achieve partially to fully covered capsules, and the polymerization reaction was observed with real-time monitoring using dynamic. light scattering (NanoDLS). The DDAB:DOPE liposomes showed stability in the tested temperature range (25-70 degrees C), whereas the results confirmed the success of the polymerization according to superficial charge (zeta potential of +66.7 +/- 1.2 mV) results and AFM images. For the liposomes that were fully coated with PAA (zeta potential of +0.3 +/- 3.9 mV), cytotoxicity was independent of the concentration of albumin. Cationic liposomes and nanocapsules of the stable liposomes coated with PAA were obtained by controlling the surface charge, which was the most important factor related to cytotoxicity. Thus, a potential, safe drug nanocarrier was successfully developed in this work. (C) 2011 Elsevier BM. All rights reserved.

引用

页码：267 / 272

页数：6

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