Impacts of chlorothalonil on denitrification and N2O emission in riparian sediments: Microbial metabolism mechanism

被引:172
作者
Su, Xiaoxuan [1 ,2 ]
Chen, Yi [1 ,2 ]
Wang, Yiyu [1 ,2 ]
Yang, Xiangyu [1 ,2 ]
He, Qiang [1 ,2 ]
机构
[1] Chongqing Univ, Key Lab Three Gorges Reservoir Reg Ecoenvironm, Minist Educ, Chongqing 400045, Peoples R China
[2] Chongqing Univ, Coll Urban Construct & Environm Engn, Chongqing 400045, Peoples R China
关键词
Riparian zone; Denitrification; Nitrous oxide; Metabolic activity; Pesticide; 3 GORGES RESERVOIR; NITROGEN-CYCLE; ELECTRON-TRANSPORT; PROCESS RATES; BUFFER ZONES; HOT MOMENTS; SOIL; NANOPARTICLES; WATER; COMMUNITIES;
D O I
10.1016/j.watres.2018.10.052
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Riparian zones can receive large amounts of nitrate, potentially contributing to water pollution. Denitrification is a major pathway to remove nitrate. Previous research on riparian denitrification focused on natural factors, but frequently neglected the roles of human activity, such as pesticide accumulations. Here, we combined field investigations and exposure experiments to reveal the responses of denitrification and N2O emission to chlorothalonil (CTN, a common pesticide) in column experiments with riparian sediments. In this study, CTN inhibited denitrification and led to nitrate accumulation in sediments. Furthermore, (TN significantly increased N2O emission by 208-377%, and this response was regulated by N2O reductase (NOS) activity rather than nosZ abundance. A mechanistic study indicated that the critical step (glyceraldehyde-3-phosphate to 3-phosphogylcerate) catalyzed by glyceraldehyde-3-phosphate dehydrogenase during microbial metabolism greatly influenced denitrification in CTN-polluted sediments. Our data also revealed that CTN declined electron donor NADH, electron transport system, and denitrifying enzyme activities during denitrification. Such responses suggested that CTN deteriorated sediment denitrification by inhibiting electron production, transport and consumption in denitrifiers. Additionally, structure equation modeling indicated that NOS was the key factor in predicting denitrification rate in CTN-polluted sediments. Overall, this is the first study to explore the effects of pesticide on denitrification and N2O emission in riparian zones at microbial metabolism level. Our results suggest that the safety threshold of CTN accumulation for inhibiting sediment denitrification is approximately 2 mg kg(-1), and imply that the wide presence of pesticides in riparian zones could impact eutrophication control of aquatic ecosystems. (C) 2018 Elsevier Ltd. All rights reserved.
引用
收藏
页码:188 / 197
页数:10
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