Molecular cloning and functional analysis of a novel Cx43 partner protein CIP150

被引：25

作者：

Akiyama, M ^{[1
]}

Ishida, N ^{[1
]}

Ogawa, T ^{[1
]}

Yogo, K ^{[1
]}

Takeya, T ^{[1
]}

机构：

[1] Nara Inst Sci & Technol, Grad Sch Biol Sci, Ikoma, Nara 6300129, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 335卷 / 04期

基金：

日本学术振兴会;

关键词：

connexin; 43; carboxyl terminal tail; partner protein; CIP150; subcellular localization; dye transfer;

D O I：

10.1016/j.bbrc.2005.08.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We identified and cloned a novel gene encoding a partner protein, CIP150, of connexin 43 (Cx43). CIP150 associates with Cx43 through its carboxyl terminal domain. Conversely, a region consisting of 16 amino acids in the juxtamembrane region (amino acids 227-242) in the carboxyl terminal tail of Cx43 was identified to be responsible for the association. A variant of Cx43 lacking this region was expressed only in a nonphosphorylated form and appeared to lose the capacity to localize to the region of cell-cell contact and dye transfer activity. When the expression of CIP150 was suppressed using small interfering RNA, the localization to the plasma membrane as well as dye transfer activity of Cx43 was significantly reduced. These results suggest that the newly identified domain is essential for the proper phosphorylation and localization of Cx43, and CIP150 is a novel partner protein targeting this domain. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：1264 / 1271

页数：8

共 30 条

[1] Refractory nature of normal human diploid fibroblasts with respect to oncogene-mediated transformation [J].