Label-free DNA detection based on whispering gallery mode optofluidic microcavity biosensor

被引：2
|
作者：
Fu, Liang [1 ]
Chen, Xiaogang [1 ]
Lu, Qijing [1 ]
Xie, Shusen [1 ]
机构：
[1] Fujian Normal Univ, Prov Key Lab Photon Technol, Minist Educ, Key Lab Optoelect Sci & Technol Med, Fuzhou 350007, Fujian, Peoples R China
来源：
NANOPHOTONICS AND MICRO/NANO OPTICS IV | 2018年 / 10823卷
基金：
中国国家自然科学基金; 中国博士后科学基金;
关键词：
Biosensor; microcavity; single strand DNA; coupling depth; SHIFT;
D O I：
10.1117/12.2501139
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
We proposed and experimentally demonstrated a sensitive, label-free, real-time and low-cost optofluidic microcavity biosensor for DNA detection. The change of whispering gallery mode (WGM) resonant wavelength is monitored by data acquisition card in real-time. The experimental results show that the biosensor can significantly distinguish the non-complementary single strand (ssDNA), single nucleotide mismatch ssDNA, and target ssDNA. In addition, a sensing mechanism based on WGM coupling depth was also experimentally demonstrated which would have potential for detection of biological and chemical analytes in the future.
引用
收藏
页数：6
相关论文
共 50 条
  • [1] The whispering gallery mode biosensor: label-free detection from virus to single protein
    Holler, S.
    Dantham, V. R.
    Keng, D.
    Kolchenko, V.
    Arnold, S.
    Mulroe, B.
    Paspaley-Grbavac, M.
    BIOSENSING AND NANOMEDICINE VII, 2014, 9166
  • [2] Label free optofluidic DNA hybridization detection based on suspended core fiber whispering gallery mode resonator
    Li, Xuegang
    Zhang, Hongxin
    Wang, Yiming
    Zhou, Xue
    Nguyen, Linh Viet
    Warren-Smith, Stephen C.
    MEASUREMENT, 2023, 222
  • [3] Optical whispering gallery mode resonators for label-free detection of water contaminants
    Berneschi, Simone
    Bettazzi, Francesca
    Giannetti, Ambra
    Baldini, Francesco
    Conti, Gualtiero Nunzi
    Pelli, Stefano
    Palchetti, Ilaria
    TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2020, 126 (126)
  • [4] Fiber optofluidic biosensor for the label-free detection of DNA hybridization and methylation based on an in-line tunable mode coupler
    Gao, Ran
    Lu, Dan-Feng
    Cheng, Jin
    Jiang, Yi
    Jiang, Lan
    Xu, Jian-Dong
    Qi, Zhi-Mei
    BIOSENSORS & BIOELECTRONICS, 2016, 86 : 321 - 329
  • [5] Whispering-gallery-mode biosensing: label-free detection down to single molecules
    Frank Vollmer
    Stephen Arnold
    Nature Methods, 2008, 5 : 591 - 596
  • [6] Label-free detection of ovarian cancer biomarkers using whispering gallery mode imaging
    Huckabay, Heath A.
    Wildgen, Sarah M.
    Dunn, Robert C.
    BIOSENSORS & BIOELECTRONICS, 2013, 45 : 223 - 229
  • [7] Whispering-gallery-mode biosensing: label-free detection down to single molecules
    Vollmer, Frank
    Arnold, Stephen
    NATURE METHODS, 2008, 5 (07) : 591 - 596
  • [8] Whispering Gallery Mode Microresonator Sensor Integrated in Exposed Core Fiber for Label-Free DNA Hybridization Detection
    Zhou, Xue
    Wang, Shankun
    Li, Xuegang
    Zhao, Yong
    Zhang, Yanan
    Nguyen, Linh Viet
    Warren-Smith, Stephen C.
    JOURNAL OF LIGHTWAVE TECHNOLOGY, 2023, 41 (13) : 4502 - 4508
  • [9] Label-free single cancer marker protein detection using a nanoplasmonic-photonic hybrid whispering gallery mode biosensor
    Holler, S.
    Danthan, V. R.
    Keng, D.
    Kolchenko, V.
    Arnold, S.
    SENSING TECHNOLOGIES FOR GLOBAL HEALTH, MILITARY MEDICINE, AND ENVIRONMENTAL MONITORING IV, 2014, 9112
  • [10] Integrating a DNA Strand Displacement Reaction with a Whispering Gallery Mode Sensor for Label-Free Mercury (II) Ion Detection
    Wu, Fengchi
    Wu, Yuqiang
    Niu, Zhongwei
    Vollmer, Frank
    SENSORS, 2016, 16 (08)
12345