Nanoleakage Distribution at Adhesive-Dentin Interfaces in 3D

被引：15

作者：

Coutinho, E. ^{[1
,2
]}

Cardoso, M. V. ^{[1
]}

Fernandes, C. P. ^{[2
]}

Neves, A. A. ^{[1
]}

Gouvea, C. V. D. ^{[2
]}

Van Landuyt, K. L. ^{[1
]}

De Munck, J. ^{[1
]}

Van Meerbeek, B. ^{[1
]}

机构：

[1] Catholic Univ Louvain, Leuven BIOMAT Res Cluster, Dept Conservat Dent, Sch Dent Oral Pathol & Maxillofacial Surg, B-3000 Louvain, Belgium

[2] Univ Fed Fluminense, Dept Prosthodont, Sch Dent, Rio De Janeiro, Brazil

来源：

JOURNAL OF DENTAL RESEARCH | 2011年 / 90卷 / 08期

关键词：

3D; FIB tomography; electron tomography; dental bonding; dentin; SCANNING-ELECTRON-MICROSCOPY; PHASE-SEPARATION; 2; MODES; EXPRESSION;

D O I：

10.1177/0022034511408430

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

In spite of its role in the degradation of tooth-biomaterial interfaces, reports on nanoleakage are largely inconsistent. The aim of this work was to assess nanoleakage patterns qualitatively and quantitatively in 3D, to determine the influence of direction, position, and inclination of the field-of-view. Therefore, we applied a gold-standard 3-step etch-and-rinse adhesive to bur-cut dentin surfaces, after which interface samples were sectioned, infiltrated with an ammoniacal silver-nitrate solution, and embedded by common TEM procedures. High-resolution 3D models of interfaces were then generated by FIB and electron tomography, following strict conditions determined by Monte Carlo simulations. Inverted images in FIB tomography disclosed morphological characteristics analogous to those revealed by TEM. Quantitative analysis revealed large variations in silver-nitrate uptake between 2D image projections in different directions. Furthermore, silver-nitrate fractions in individual 2D image projections were seldom related to the total 3D volumetric fraction. Electron tomography showed that inclination also affected the morphology of silver-nitrate patterns. In conclusion, conventional nanoleakage evaluation is heavily influenced by direction, position, and inclination of the field-of-view, and thus may contain artifacts.

引用

页码：1019 / 1025

页数：7

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