MXene-based cytosensor for the detection of HER2-positive cancer cells using CoFe2O4@Ag magnetic nanohybrids conjugated to the HB5 aptamer

被引:65
作者
Vajhadin, Fereshteh [1 ]
Mazloum-Ardakani, Mohammad [1 ]
Shahidi, Maryamsadat [2 ]
Moshtaghioun, Seyed Mohammad [3 ]
Haghiralsadat, Fateme [4 ,5 ]
Ebadi, Azar [1 ]
Amini, Abbas [6 ,7 ]
机构
[1] Yazd Univ, Fac Sci, Dept Chem, Yazd 89195741, Iran
[2] Shahid Sadoughi Univ Med Sci & Hlth Serv, Sch Med, Dept Clin Biochem, Yazd, Iran
[3] Yazd Univ, Fac Sci, Dept Biol, Yazd, Iran
[4] Shahid Sadoughi Univ Med Sci, Med Nanotechnol & Tissue Engn Res Ctr, Yazd Reprod Sci Inst, Yazd, Iran
[5] Shahid Sadoughi Univ Med Sci, Sch Paramed, Dept Adv Med Sci & Technol, Yazd, Iran
[6] Australian Coll Kuwait, Dept Mech Engn, Safat 13015, Kuwait
[7] Western Sydney Univ, Ctr Infrastruct Engn, Penrith, NSW 2751, Australia
基金
美国国家科学基金会;
关键词
MXene; Electrochemical cytosensor; HER2-positive cancer cells; Magnetic nanoparticles; Aptamer; Circulating tumor cells; NANOPARTICLES; ADSORPTION; BIOSENSOR; DNA;
D O I
10.1016/j.bios.2021.113626
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
MXenes are a new class of conductive two-dimensional material which have received growing attention in biosensing for their significant surface area and unique surface chemistry. Here, gold electrodes were modified with MXene nanosheets of about 2 nm thickness and 1.5 mu m lateral size for the electrochemical detection of tumor cells. An HB5 aptamer with high selectivity for HER-2 positive cells was immobilized on the MXene layers via electrostatic interactions. To minimize electrode biofouling with blood matrix, magnetic separation of HER-2 positive circulating tumor cells was carried out using CoFe2O4@Ag magnetic nanohybrids bonded to the HB5. The formation of sandwich-like structures between the magnetically captured cells and the functionalized MXene electrodes effectively shields the electron transfer of a redox probe, enabling quantitative cell detection using the change in current. This label-free MXene-based cytosensor platform yielded a wide linear range of 10(2)-10(6) cells/ mL, low detection limit of 47 cells/mL, and good sensitivity and selectivity in the detection of HER2-posetive cells in blood samples. The presented aptacytosensor demonstrates the great potential of using CoFe2O4@Ag magnetic nanohybrids and MXenes to monitor cancer progression via circulating tumor cells in blood at low cost.
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页数:9
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