Detection of Polymorphism in Exon 2 of Toll-like Receptor 4 Gene of Indian Buffaloes Using PCR-SSCP Technique

Dubey, P.K., Selvakumar, M., Kathiravan, P., Yadav, N., Mishra, B.P. and Kataria, R.S. 2010. Detection of polymorphism in exon 2 of toll-like receptor 4 gene of Indian buffaloes using PCR-SSCP technique. J. Appl. Anim. Res., 37: 265-268. Toll-like receptor 4 (TLR4) sequence of Indian buffalo population was analysed for the detection of variation within exon2 region. 81 DNA samples representing six different buffalo breeds viz. Jaffarabadi, Marathwada, Toda, Pandharpuri, Mehsana and Chilika were amplified using the primers specific to exon 2 and adjoining intronic sequence. PCR products when resolved on non-denaturing polyacrylamide gel electrophoresis (PAGE) yielded two different variants A and B with almost equal frequency of 0.531 and 0.469, respectively, which further varied among the buffalo breeds investigated. Sequencing analysis of buffalo TLR 4 exon 2 revealed 167 nucleotides, coding for 55 amino acids as in other livestock species. Cattle and buffalo had 100% amino acid homology with few changes as compared to sheep and goat. The variants reported in the present study can be used as candidate markers for economically important traits in buffalo.