Evaluation of Human Osteosarcoma Cell Line Genotoxicity Effects of Mineral Trixoide Aggregate and Calcium Silicate Cements

被引：19
作者：
Ding, Shinn-Jyh [1 ]
Kao, Chia-Tze [2 ]
Chen, Chih-Lin [1 ]
Shie, Ming-You [1 ]
Huang, Tsui-Hsien [1 ]
机构：
[1] Chung Shan Med Univ, Inst Oral Biol & Biomat, Taichung 402, Taiwan
[2] Chung Shan Med Univ, Inst Dent Sch, Taichung 402, Taiwan
来源：
JOURNAL OF ENDODONTICS | 2010年 / 36卷 / 07期
关键词：
Calcium silicate; DNA precipitation assay; genotoxicity; mineral trioxide aggregate; TRIOXIDE AGGREGATE; SEALING ABILITY; BIOCOMPATIBILITY; CYTOTOXICITY; RESORPTION; REPAIR;
D O I：
10.1016/j.joen.2010.03.039
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Introduction: Mineral trioxide aggregate (MTA) and calcium silicate (CS) cements exhibit acceptable levels of cytocompatibility. The aim of the present study was to evaluate MTA and CS cement genotoxicity in a human osteosarcoma cell line (MG63). Methods: A mitochondrial colorimetric assay was used to evaluate the MG63 survival rate. A DNA precipitation assay was used to detect the MG63 DNA damage after contact with MTA or CS cement extracts. Results: The results showed that MTA and CS are cytocompatible with MG63 cells. There was no significant difference in the survival rate with MTA and CS materials (P > .05). Neither MTA nor CS cements causing MG63 cell DNA damage showed significant genotoxicity (P > .05). Conclusions: Both MTA and CS cements are compatible with MG63 cells, and they are not cancer-causing agents. (J Endod 2010;36:1158-1162)
引用
收藏
页码：1158 / 1162
页数：5
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