Silica-Coated Gold Nanostars for Combined Surface-Enhanced Raman Scattering (SERS) Detection and Singlet-Oxygen Generation: A Potential Nanoplatform for Theranostics

被引：183

作者：

Fales, Andrew M. ^{[1
,2
]}

Yuan, Hsiangkuo ^{[1
,2
]}

Vo-Dinh, Tuan ^{[1
,2
,3
]}

机构：

[1] Duke Univ, Fitzpatrick Inst Photon, Durham, NC 27708 USA

[2] Duke Univ, Dept Biomed Engn, Durham, NC 27708 USA

[3] Duke Univ, Dept Chem, Durham, NC 27708 USA

来源：

LANGMUIR | 2011年 / 27卷 / 19期

基金：

美国国家卫生研究院;

关键词：

PHOTODYNAMIC THERAPY; METHYLENE-BLUE; NANOPARTICLE PLATFORMS; SPECTROSCOPY; PHOTOSENSITIZERS; PARTICLES; GROWTH; TUMORS; MICE;

D O I：

10.1021/la202602q

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This paper reports the synthesis and characterization of surface-enhanced Raman scattering (SERS) label-tagged gold nanostars, coated with a silica shell containing methylene blue photosensitizing drug for singlet-oxygen generation. To our knowledge, this is the first report of nanocomposites possessing a combined capability for SERS detection and singlet-oxygen generation for photodynamic therapy. The gold nanostars were tuned for maximal absorption in the near-infrared (NIR) spectral region and tagged with a NIR dye for surface-enhanced resonance Raman scattering (SERRS). Silica coating was used to encapsulate the photosensitizer methylene blue in a shell around the nanoparticles. Upon 785 nm excitation, SERS from the Raman dye is observed, while excitation at 633 nm shows fluorescence from methylene blue. Methylene-blue-encapsulated nanoparticles show a significant increase in singlet-oxygen generation as compared to nanoparticles synthesized without methylene blue. This increased singlet-oxygen generation shows a cytotoxic effect on BT549 breast cancer cells upon laser irradiation. The combination of SERS detection (diagnostic) and singlet-oxygen generation (therapeutic) into a single platform provides a potential theranostic agent.

引用

页码：12186 / 12190

页数：5

共 35 条

[1] Rhodamine 6G-SiO2 hybrids:: A photoluminescence study [J].

Anedda, A ;

Carbonaro, CM ;

Clemente, F ;

Corpino, R ;

Grandi, S ;

Magistris, A ;

Mustarelli, PC .

JOURNAL OF NON-CRYSTALLINE SOLIDS, 2005, 351 (21-23) :1850-1854

[2] Ultrabright Fluorescent Mesoporous Silica Nanoparticles [J].

Cho, Eun-Bum ;

Volkov, Dmytro O. ;

Sokolov, Igor .

SMALL, 2010, 6 (20) :2314-2319

[3] Spectroscopic tags using dye-embedded nanoparticles and surface-enhanced Raman scattering [J].

Doering, WE ;

Nie, SM .

ANALYTICAL CHEMISTRY, 2003, 75 (22) :6171-6176

[4] SERS as a foundation for nanoscale, optically detected biological labels [J].

Doering, William E. ;

Piotti, Marcelo E. ;

Natan, Michael J. ;

Freeman, R. Griffith .

ADVANCED MATERIALS, 2007, 19 (20) :3100-3108

[5] Photodynamic therapy for cancer [J].

Dolmans, DEJGJ ;

Fukumura, D ;

Jain, RK .

NATURE REVIEWS CANCER, 2003, 3 (05) :380-387

[6] PHOTOSENSITIZERS - THERAPY AND DETECTION OF MALIGNANT-TUMORS [J].

DOUGHERTY, TJ .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1987, 45 (06) :879-889

[7] Surface-enhanced Raman scattering spectroscopy via gold nanostars [J].

Esenturk, E. Nalbant ;

Walker, A. R. Hight .

JOURNAL OF RAMAN SPECTROSCOPY, 2009, 40 (01) :86-91

[8] Highly Controlled Silica Coating of PEG-Capped Metal Nanoparticles and Preparation of SERS-Encoded Particles [J].

Fernandez-Lopez, Cristina ;

Mateo-Mateo, Cintia ;

Alvarez-Puebla, Ramon A. ;

Perez-Juste, Jorge ;

Pastoriza-Santos, Isabel ;

Liz-Marzan, Luis M. .

LANGMUIR, 2009, 25 (24) :13894-13899

[9] Methylene blue-encapsulated phosphonate-terminated silica nanoparticles for simultaneous in vivo imaging and photodynamic therapy [J].

He, Xiaoxiao ;

Wu, Xu ;

Wang, Kemin ;

Shi, Bihua ;

Hai, Luo .

BIOMATERIALS, 2009, 30 (29) :5601-5609

[10] SURFACE-ENHANCED RESONANCE RAMAN-SPECTROSCOPY OF RHODAMINE-6G ADSORBED ON COLLOIDAL SILVER [J].

HILDEBRANDT, P ;

STOCKBURGER, M .

JOURNAL OF PHYSICAL CHEMISTRY, 1984, 88 (24) :5935-5944

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