Novel type of fimbriae encoded by the large plasmid of sorbitol-fermenting enterohemorrhagic Escherichia coli O157:H-

被引:85
作者
Brunder, W
Khan, AS
Hacker, J
Karch, H
机构
[1] Univ Wurzburg, Inst Hyg & Mikrobiol, D-97080 Wurzburg, Germany
[2] Univ Wurzburg, Inst Mol Infekt Biol, D-97070 Wurzburg, Germany
关键词
D O I
10.1128/IAI.69.7.4447-4457.2001
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Sorbitol-fermenting (SF) enterohemorrhagic Escherichia coli (EHEC) O157:H- have emerged as important causes of diarrheal diseases and the hemolytic-uremic syndrome in Germany. In this study, we characterized a 32-kb fragment of the plasmid of SF EHEC O157:H-, pSFO157, which differs markedly from plasmid pO157 of classical non-sorbitol-fermenting EHEC O157:H7, We found a cluster of six genes, termed sfgA, sfpH, sfpC, sfpD, sfpJ, and sfpG, which mediate mannose-resistant hemagglutination and the expression of fimbriae. sfp genes are similar to the pag genes, encoding P-fimbriae of uropathogenic E. coli, but the sfg cluster lacks homologues of genes encoding subunits of a tip fibrillum as well as regulatory genes, The major pilin, SfpA, despite its similarity to PapA, does not cluster together with known PapA alleles in a phylogenetic tree but is structurally related to the PmpA pilin of Proteus mirabilis. The putative adhesin gene sfpG, responsible for the hemagglutination phenotype, shows significant homology neither to papG nor to other known sequences. Sfp fimbriae are 3 to 5 nm in diameter, in contrast to P-fimbriae, which are 7 nm in diameter, PCR analyses showed that the sfp gene cluster is a characteristic of SF EHEC O157:H- strains and is not present in other EHEC isolates, diarrheagenic E. coli, or other Enterobacteriaceae. The sfp gene cluster is Ranked by two blocks of insertion sequences and an origin of plasmid replication, indicating that horizontal gene transfer may have contributed to the presence of Sfp fimbriae in SF EHEC O157:H-.
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页码:4447 / 4457
页数:11
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共 61 条
  • [1] A large outbreak of hemolytic uremic syndrome caused by an unusual sorbitol-fermenting strain of Escherichia coli O157:H-
    Ammon, A
    Petersen, LR
    Karch, H
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1999, 179 (05) : 1274 - 1277
  • [2] BIOGENESIS OF ESCHERICHIA-COLI PAP PILI - PAPH, A MINOR PILIN SUBUNIT INVOLVED IN CELL ANCHORING AND LENGTH MODULATION
    BAGA, M
    NORGREN, M
    NORMARK, S
    [J]. CELL, 1987, 49 (02) : 241 - 251
  • [3] Isolation and characterization of sorbitol-fermenting Shiga toxin (Verocytotoxin)-producing Escherichia coli O157:H-strains in the Czech Republic
    Bielaszewska, M
    Schmidt, H
    Karmali, MA
    Khakhria, R
    Janda, J
    Bláhová, K
    Karch, H
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (07) : 2135 - 2137
  • [4] Cattle can be a reservoir of sorbitol-fermenting Shiga toxin-producing Escherichia coli O157:H- strains and a source of human diseases
    Bielaszewska, M
    Schmidt, H
    Liesegang, A
    Prager, R
    Rabsch, W
    Tschäpe, H
    Cízek, A
    Janda, J
    Bláhová, K
    Karch, H
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (09) : 3470 - 3473
  • [5] BIJLSMA IGW, 1995, MICROBIOL-SGM, V141, P1349, DOI 10.1099/13500872-141-6-1349
  • [6] TYPE-1 FIMBRIAE MUTANTS OF ESCHERICHIA-COLI K12 - CHARACTERIZATION OF RECOGNIZED AFIMBRIATE STRAINS AND CONSTRUCTION OF NEW FIM DELETION MUTANTS
    BLOMFIELD, IC
    MCCLAIN, MS
    EISENSTEIN, BI
    [J]. MOLECULAR MICROBIOLOGY, 1991, 5 (06) : 1439 - 1445
  • [7] The large plasmids of Shiga-toxin-producing Escherichia coli (STEC) are highly variable genetic elements
    Brunder, W
    Schmidt, H
    Frosch, M
    Karch, H
    [J]. MICROBIOLOGY-UK, 1999, 145 : 1005 - 1014
  • [8] KatP, a novel catalase-peroxidase encoded by the large plasmid of enterohaemorrhagic Escherichia coli O157:H7
    Brunder, W
    Schmidt, H
    Karch, H
    [J]. MICROBIOLOGY-SGM, 1996, 142 : 3305 - 3315
  • [9] EspP, a novel extracellular serine protease of enterohaemorrhagic Escherichia coli O157:H7 cleaves human coagulation factor V
    Brunder, W
    Schmidt, H
    Karch, H
    [J]. MOLECULAR MICROBIOLOGY, 1997, 24 (04) : 767 - 778
  • [10] STRUCTURAL POLYMORPHISM OF BACTERIAL ADHESION PILI
    BULLITT, E
    MAKOWSKI, L
    [J]. NATURE, 1995, 373 (6510) : 164 - 167