Direct Genotyping of Toxoplasma gondii in Ocular Fluid Samples from 20 Patients with Ocular Toxoplasmosis: Predominance of Type II in France

被引：48

作者：

Fekkar, A. ^{[1
,2
,3
]}

Ajzenberg, D. ^{[4
,5
]}

Bodaghi, B. ^{[6
]}

Touafek, F. ^{[1
]}

Le Hoang, P. ^{[6
]}

Delmas, J. ^{[7
]}

Robert, P. Y. ^{[7
]}

Darde, M. L. ^{[4
,5
]}

Mazier, D. ^{[1
,2
,3
]}

Paris, L. ^{[1
]}

机构：

[1] Grp Hosp Pitie Salpetriere, AP HP, Serv Parasitol Mycol, F-75013 Paris, France

[2] Univ Paris 06, UMR S945, F-75013 Paris, France

[3] INSERM, U945, F-75013 Paris, France

[4] Univ Limoges, Fac Med, EA3174 NETEC, Lab Parasitol Mycol, F-87031 Limoges, France

[5] Ctr Hosp Univ Dupuytren, Toxoplasmose Toxoplasma Biol Resource Ctr BRC, CNR, F-87042 Limoges, France

[6] Grp Hosp Pitie Salpetriere, AP HP, Serv Ophtalmol, F-75013 Paris, France

[7] Ctr Hosp Univ Dupuytren, Serv Ophtalmol, F-87042 Limoges, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 04期

关键词：

FREE-RANGE CHICKENS; REAL-TIME PCR; CONGENITAL TOXOPLASMOSIS; CLINICAL FINDINGS; SOUTHERN BRAZIL; MULTIPLEX PCR; STRAINS; DISEASE; INFECTION; COLOMBIA;

D O I：

10.1128/JCM.02196-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We report the direct genotyping analysis of Toxoplasma gondii in ocular samples collected from 20 patients, as well as associated clinical and epidemiological data. This work was aimed at better understanding the impact of genotypes of Toxoplasma gondii strains on toxoplasmic retinochoroiditis. For this purpose, we studied the aqueous humor (AH) or vitreous humor (VH) of 20 patients presenting with ocular toxoplasmosis (OT) in 2 hospitals in France. Genetic characterization was obtained with microsatellite markers in a multiplex PCR assay. In contrast to the results of previous studies, we found no association between atypical Toxoplasma gondii genotypes and the occurrence of OT. Considering the local epidemiological data, our OT patients seemed to be infected more frequently by ordinary type II strains found in the environment. In conclusion, direct genotyping of Toxoplasma gondii strains from aqueous or vitreous humor showed a predominance of the type II genotype in ocular toxoplasmosis; this may be due to a high exposure rate of this genotype in humans.

引用

页码：1513 / 1517

页数：5

共 31 条

[1] Multiplex PCR for typing strains of Toxoplasma gondii [J].

Ajzenberg, D ;

Dumètre, A ;

Dardé, ML .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (04) :1940-1943

[2] Genetic diversity, clonality and sexuality in Toxoplasma gondii [J].

Ajzenberg, D ;

Bañuls, AL ;

Su, C ;

Dumètre, A ;

Demar, M ;

Carme, B ;

Dardé, ML .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2004, 34 (10) :1185-1196

[3] Genotype of 86 Toxoplasma gondii isolates associated with human congenital toxoplasmosis, and correlation with clinical findings [J].

Ajzenberg, D ;

Cogné, N ;

Paris, L ;

Bessières, MH ;

Thulliez, P ;

Filisetti, D ;

Pelloux, H ;

Marty, P ;

Dardé, ML .

JOURNAL OF INFECTIOUS DISEASES, 2002, 186 (05) :684-689

[4] Genotype of 88 Toxoplasma gondii Isolates Associated with Toxoplasmosis in Immunocompromised Patients and Correlation with Clinical Findings [J].

Ajzenberg, Daniel ;

Yera, Helene ;

Marty, Pierre ;

Paris, Luc ;

Dalle, Frederic ;

Menotti, Jean ;

Aubert, Dominique ;

Franck, Jacqueline ;

Bessieres, Marie-Helene ;

Quinio, Dorothee ;

Pelloux, Herve ;

Delhaes, Laurence ;

Desbois, Nicole ;

Thulliez, Philippe ;

Robert-Gangneux, Florence ;

Kauffmann-Lacroix, Catherine ;

Pujol, Sophie ;

Rabodonirina, Meja ;

Bougnoux, Marie-Elisabeth ;

Cuisenier, Bernadette ;

Duhamel, Chantal ;

Duong, Thanh Hai ;

Filisetti, Denis ;

Flori, Pierre ;

Gay-Andrieu, Francoise ;

Pratlong, Francine ;

Nevez, Gilles ;

Totet, Anne ;

Carme, Bernard ;

Bonnabau, Henri ;

Darde, Marie-Laure ;

Villena, Isabelle .

JOURNAL OF INFECTIOUS DISEASES, 2009, 199 (08) :1155-1167

[5] Bilateral retinochoroiditis caused by an atypical strain of Toxoplasma gondii [J].

Bottos, J. ;

Miller, R. H. ;

Belfort, R. N. ;

Macedo, A. C. ;

Belfort, R., Jr. ;

Grigg, M. E. .

BRITISH JOURNAL OF OPHTHALMOLOGY, 2009, 93 (11) :1546-1550

[6] Severe acquired toxoplasmosis in immunocompetent adult patients in French Guiana [J].

Carme, B ;

Bissuel, F ;

Ajzenberg, D ;

Bouyne, R ;

Aznar, C ;

Demar, M ;

Bichat, S ;

Louvel, D ;

Bourbigot, AM ;

Peneau, C ;

Neron, P ;

Dardé, ML .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (11) :4037-4044

[7]

De Salvador-Guillouet Francine, 2006, J Infect, V53, pe47, DOI 10.1016/j.jinf.2005.10.026

[8] Biologic and molecular characterization of Toxoplasma gondii isolates from pigs from Portugal [J].

de Sousa, S ;

Ajzenberg, D ;

Canada, N ;

Freire, L ;

da Costa, JMC ;

Dardé, ML ;

Thulliez, P ;

Dubey, JP .

VETERINARY PARASITOLOGY, 2006, 135 (02) :133-136

[9] Screening by Ophthalmoscopy for Toxoplasma retinochoroiditis in Colombia [J].

de-la-Torre, Alejandra ;

Gonzalez, Gonzaga ;

Diaz-Ramirez, Julieth ;

Gomez-Marin, Jorge Enrique .

AMERICAN JOURNAL OF OPHTHALMOLOGY, 2007, 143 (02) :354-356

[10] Fatal outbreak of human toxoplasmosis along the maroni river:: Epidemiological, clinical, and parasitological aspects [J].

Demar, Magalie ;

Ajzenberg, Daniel ;

Maubon, Danielle ;

Djossou, Felix ;

Panchoe, Dayanand ;

Punwasi, Widya ;

Valery, Nasser ;

Peneau, Christian ;

Daigre, Jean-Louis ;

Aznar, Christine ;

Cottrelle, Benoit ;

Terzan, Laurence ;

Darde, Marie-Laure ;

Carme, Bernard .

CLINICAL INFECTIOUS DISEASES, 2007, 45 (07) :E88-E95

← 1 2 3 4 →