The pre-event warm-up or "priming" routine for optimising cycling performance is not well-defined or uniform to a specific event. We aimed to determine the effects of varying the intensity of priming on 3km cycling performance. Ten endurance-trained male cyclists completed four 3km time-trials (TT) on four separate occasions, each preceded by a different priming strategy including "self-selected" priming and three intermittent priming strategies incorporating 10min of constant-load cycling followed by 5x10s bouts of varying relative intensity (100% and 150% of peak aerobic power, W-peak, and all-out priming). The self-selected priming trial (379 +/- 44W) resulted in similar mean power during the 3km TT to intermittent priming at 100% (376 +/- 45W; -0.7%; unclear) and 150% (374 +/- 48W; -1.5%, unclear) of W-peak, but significantly greater than all-out priming (357 +/- 45W; -5.8%, almost certainly harmful). Differences between intermittent and self-selected priming existed with regards to heart rate (6.2% to 11.5%), blood lactate (-22.9% to 125%) and VO2 kinetics (-22.9% to 8.2%), but these were not related to performance outcomes. In conclusion, prescribed intermittent priming strategies varying in intensity did not substantially improve 3km TT performance compared to self-selected priming.