Proteome composition and profiling of bioactive peptides of edible Antheraea assamensis pupae by sequential enzymatic digestion and kinetic modeling of in vitro gastrointestinal digestion

被引：11

作者：

Sarkar, Preeti ^{[1
]}

Valacchi, Giuseppe ^{[2
,3
]}

Duary, Raj Kumar ^{[1
,4
]}

机构：

[1] Tezpur Univ, Dept Food Engn & Technol, Tezpur 784028, Assam, India

[2] NC State Univ, Plants Human Hlth Inst, NC Res Campus, Kannapolis, NC 28081 USA

[3] Univ Ferrara, Dept Life Sci & Biotechnol, Ferrara, Italy

[4] Banaras Hindu Univ BHU, Inst Agr Sci, Dept Dairy Sci & Food Technol, Varanasi 221005, Uttar Pradesh, India

来源：

EUROPEAN FOOD RESEARCH AND TECHNOLOGY | 2022年 / 248卷 / 02期

关键词：

Insect hydrolysate; Papain; Thermolysin; Gastrointestinal digestion; Free amino acids; Enzyme kinetics; ACE-INHIBITORY PEPTIDES; TENEBRIO-MOLITOR; SILKWORM PUPAE; BOMBYX-MORI; ANTIOXIDANT; HYDROLYSIS; INSECTS; FOOD; IDENTIFICATION; CONVERSION;

D O I：

10.1007/s00217-021-03882-z

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Among several foods-derived peptides/protein hydrolysates tested to treat fluctuation in oxidative stress, Angiotensin-I converting enzyme (ACE) and dipeptidyl peptidase IV (DPP-IV) activities linked with physiological complications of type II diabetes and hypertension, only limited numbers sourced from insects have been implemented. Aim of this study is to profile the indigenous Muga (Antheraea assamensis) pupae proteome and evaluate the suitable parameters to procure ACE inhibitory, DPP-IV inhibitory and antioxidative peptide mix with suitable enzymatic interventions. Total 72 distinct proteins distributed in 22 insect protein families were identified through MS/MS analysis. Aldehyde dehydrogenase (6.79%), pupae cuticle protein (17.02%) and haemolymph protein (9.03%) were the most abundant enzymatic and non-enzymatic protein families. The highest ACE inhibition and DPPH scavenging activities were demonstrated by Alcalase (H1) and Papain (H3) hydrolysates at 3.0% enzyme-substrate (E/S) ratio with incubation of 3 and 5 h, respectively. The Flavourzyme (H5) and Papain (H6) hydrolysates at 2.3% E/S for 5 and 3 h incubations resulted in the best DPP-IV inhibition activity. Further, Flavourzyme (H2) and Thermolysin (H4) hydrolysates with 3.0 E/S for 5 and 2 h incubation resulted in higher ABTS scavenging activity. The respective potential activities of H1 and H3 increased while H4 and H5 were retained after simulated gastrointestinal digestion. Liquid chromatography, free amino acids and molecular mass analysis further supported variation between the profiles of non-hydrolysed and hydrolysed samples under enzymatic and simulated gastrointestional conditions. Gastrointestinal digests of protein extract, H1 and H3 displayed an uncompetitive, mixed and competitive inhibiting pattern towards ACE. While gastrointestinal digests of H5 and H6 revealed an uncompetitive and non-competitive DPP-IV inhibition. The bioactive protein hydrolysates from edible A. assamensis pupae generated using enzymatic digestion can be an effective supplement for functional or nutraceutical food formulations. [GRAPHICS] .

引用

页码：357 / 379

页数：23