Variances in the mRNA expression profile of TGF-β1-3 isoforms and its TGF-βRI-III receptors during cyclosporin a treatment of psoriatic patients

被引：9
作者：
Michalska-Bankowska, Anna [1 ]
Wcislo-Dziadecka, Dominika [2 ]
Grabarek, Benjamin [3 ]
Brzezinska-Wcislo, Ligia [1 ]
Mazurek, Urszula [3 ]
Salwowska, Natalia [1 ]
Bankowski, Miroslaw [4 ]
机构：
[1] Med Univ Silesia, Sch Med, Chair & Dept Dermatol, Katowice, Poland
[2] Med Univ Silesia, Dept Skin Struct Studies, Chair Cosmetol, Sch Pharm,Div Lab Med Sosnowiec, Katowice, Poland
[3] Med Univ Silesia, Chair & Dept Mol Biol, Sch Pharm, Div Lab Med Sosnowiec, Katowice, Poland
[4] Dist Hlth Care Ctr, Dept Neurol, Czeladz, Poland
来源：
POSTEPY DERMATOLOGII I ALERGOLOGII | 2018年 / 35卷 / 05期
关键词：
mRNA; transforming growth factor beta 1-3; psoriasis; cyclosporin A; TYPE-2; DIABETES-MELLITUS; HUMAN DERMAL FIBROBLASTS; GROWTH-FACTOR BETA-1; METABOLIC SYNDROME; TRANSFORMING GROWTH-FACTOR-BETA-1; DOWN-REGULATION; STEM-CELLS; DISEASE; VULGARIS; COMORBIDITIES;
D O I：
10.5114/ada.2018.77242
中图分类号：
R392 [医学免疫学];
学科分类号：
100102 ;
摘要：
Introduction: Psoriasis is a chronic, immunologic, multi-factor inflammatory skin disease, strongly associated with a higher level of a number of cytokines, such as isoforms of transforming growth factor beta (TGF-(beta 1-3) and its receptors (TGF-beta RI-III). One of the most popular and important drugs used to treat this disease is cyclosporin A (CsA). Aim: The aim of this study was to investigate the expression of genes encoding the transforming growth factor (TGF)-beta isoforms and receptors of the cytokine TGF-beta Rs in psoriatic patients during an 84-day long observation of the effects of cyclosporin A therapy. It made an attempt to determine the usefulness of testing mRNA expression of TGF-beta 1-3 and its receptors TGF-beta RI-III as the supplementary molecular markers of lost sensitivity to the medicine. Material and methods: The study group consisted of 32 patients with psoriasis (20 men and 12 women) treated with cyclosporin A. The changes in expression patterns of TGF-beta 1-3 and TGF-beta RI-III were performed by real-time quantitative reverse transcription PCR (RTqPCR). Results: The expression of TGF-beta 1-3 and TGF-beta RI-III were detected in the whole period of therapy with CsA. Changes in transcriptional activities of TGF-beta 1-3 and TGF-beta RI-III during pharmacotherapy were observed. Differences in the expression of these genes were found before and after 42 and 84 days of using CsA. Conclusions: The changes in expression profiles of TGF-beta 1-3 and TGF-beta RI-III during CsA therapy can be a useful molecular marker of lost sensitivity to the medicine.
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页码：502 / 509
页数：8
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