SECONDARY METABOLITE PRODUCTION IN HYPERICUM PERFORATUM L. CELL SUSPENSIONS UPON ELICITATION WITH FUNGAL MYCELIA FROM ASPERGILLUS FLAVUS

被引：21

作者：

Gadzovska-Simic, Sonja ^{[1
]}

Tusevski, O. ^{[1
]}

Antevski, S. ^{[1
]}

Atanasova-Pancevska, Natalija ^{[2
]}

Petreska, Jasmina ^{[3
]}

Stefova, Marina ^{[3
]}

Kungulovski, D. ^{[2
]}

Spasenoski, M. ^{[1
]}

机构：

[1] Univ Ss Cyril & Methodius, Fac Nat Sci & Math, Lab Plant Cell & Tissue Culture, Skopje 1000, Macedonia

[2] Univ Ss Cyril & Methodius, Fac Nat Sci & Math, Inst Biol, Microbiol Lab, Skopje 1000, Macedonia

[3] Univ Ss Cyril & Methodius, Fac Nat Sci & Math, Inst Chem, Analyt Chem Lab, Skopje 1000, Macedonia

来源：

ARCHIVES OF BIOLOGICAL SCIENCES | 2012年 / 64卷 / 01期

关键词：

Anthocyanins; Aspergillus flavus; elicitation; flavanols; flavonols; hypericin; Hypericum perforatum L; naphtodianthrones; phenolics; phenylpropanoids; ST-JOHNS-WORT; CULTURES; QUANTIFICATION; IDENTIFICATION; INDUCTION; SYSTEMS; CALLI;

D O I：

10.2298/ABS1201113G

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We investigated the production of phenylpropanoids (phenolic compounds, flavanols, flavonols and anthocyanins) and naphtodianthrones (hypericins) in elicited Hypericum perforatum L. cell suspensions. To determine whether secondary metabolite production could be enhanced, Hypericum cell suspensions were exposed to mycelia extract from the fungus Aspergillus flavus. Elicited Hypericum cell suspension cultures displayed reduced growth and viability and a modification of secondary metabolites production. Anthocyanins were only stimulated in fungal-elicited cell suspensions. Secondary metabolite production in elicited Hypericum cells revealed an antagonism between the flavonoid/naphtodianthrone and anthocyanin pathways. The data suggest a modification of the channeling of the phenylpropanoid compounds. Together, these results represent useful data for monitoring the channeling in different secondary metabolite pathways during the scaled-up production of naphtodianthrones for medicinal uses.

引用

页码：113 / 121

页数：9

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