Study of the BPP7a Peptide and its β-Cyclodextrin Complex: Physicochemical Characterization and Complete Sequence Specific NMR Assignments

被引：7

作者：

Lula, Ivana ^{[1
]}

de Sousa, Frederico B. ^{[1
]}

Denadai, Angelo M. L. ^{[1
,3
]}

Ianzer, Danielle ^{[2
,4
]}

de Camargo, Antonio C. M. ^{[4
]}

Santos, Robson A. S. ^{[2
]}

Sinisterra, Ruben D. ^{[1
]}

机构：

[1] Univ Fed Minas Gerais, Dept Quim, Inst Ciencias Exatas, BR-31270901 Belo Horizonte, MG, Brazil

[2] Univ Fed Minas Gerais, Dept Fisiol & Biofis, Inst Ciencias Biol, BR-31270901 Belo Horizonte, MG, Brazil

[3] Ctr Fed Educ Tecnol CEFET MG, BR-35183006 Timoteo, MG, Brazil

[4] Ctr Appl Toxinol CAT CEPID, Inst Butanta, BR-05503900 Sao Paulo, Brazil

来源：

JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY | 2011年 / 22卷 / 09期

关键词：

bradykinin potentiating peptide; p-Glu(1)Asp(2)Gly(3)Pro(4)Ile(5)Pro(6)Pro(7); beta-cyclodextrin; association complex; NMR; HR-DOSY; ANGIOTENSIN-CONVERTING ENZYME; BRADYKININ-POTENTIATING PEPTIDES; BOTHROPS-JARARACA VENOM; FIELD GRADIENT; DIFFUSION MEASUREMENTS; CIRCULAR-DICHROISM; SPECTROSCOPY; ASSOCIATION; INHIBITORS; PROTEINS;

D O I：

10.1590/S0103-50532011000900020

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The BPP7a heptapeptide, p-Glu(1)Asp(2)Gly(3)Pro(4)Ile(5)Pro(6)Pro(7), forms an association complex with beta-cyclodextrin in a 1: 1 molar ratio. The peptide and its complex were characterized by circular dichroism (CD) and isothermal titration calorimetry (ITC), which showed a very weak interaction between the beta-cyclodextrin and the peptide. Assignments of all hydrogen resonances of the peptide alone and as a complex were made using H-1 nuclear magnetic resonance (NMR) experiments at 400 and 600 MHz. High resolution diffusion ordered spectroscopy (HR-DOSY) experiments were carried out to establish the self-aggregation state of BPP7a. It was also shown that the beta-cyclodextrin breaks the molecular clusters leading to complex formation. In addition, the anti-hypertensive activity of the BPP7a/beta-cyclodextrin complex was evaluated in spontaneous hypertensive rats (SHR), showing increased activity compared to that of pure BPP7a.

引用

页码：1765 / 1773

页数：9

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