Determination of rosmarinic acid in sage and borage leaves by high-performance liquid chromatography with different detection methods

被引：51
作者：
Bandoniene, D
Murkovic, M
Venskutonis, PR
机构：
[1] Kaunas Univ Technol, Dept Food Technol, LT-3028 Kaunas, Lithuania
[2] Graz Univ Technol, Inst Food Chem & Technol, A-8010 Graz, Austria
来源：
JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2005年 / 43卷 / 07期
关键词：
D O I：
10.1093/chromsci/43.7.372
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Rosmarinic acid is separated and identified on the basis of high-performance liquid chromatography (HPLC)-UV-mass spectrometry data in 80% methanol in water extracts from the leaves of Salvia species (S. officinalis, S. glutinosa, S. aethiopis, S. sclarea, and Borago officinalis) as a dominant radical scavenger towards the 2,2′-diphenyl-1-picrylhydrazyl (DPPH•) stable radical in HPLC-DPPH• system. The content of rosmarinic acid in the plants is calibrated and quantitated from chromatograms obtained by UV detection at 280 nm. The concentration ranges from 13.3 to 47.3 mg of the phenolic acid per gram dried leaves of all plants is tested. S. glutinosa and S. sclarea have the highest concentration of rosmarinic acid. The amount of rosmarinic acid in borage leaves is similar compared with Salvia officinalis (15 mg/g). The HPLC-DPPH• system is calibrated for quantitative DPPH• scavenging assessment of rosmarinic acid. The results reveal excellent correlation (r 2 = 0.98) between the rosmarinic acid concentration and antiradical activity.
引用
收藏
页码：372 / 376
页数：5
相关论文
共 60 条
  • [1] HPLC/DAD analysis of phenolic compounds from lavender and its application to quality control
    Areias, FM
    Valentao, P
    Andrade, PB
    Moreira, MM
    Amaral, J
    Seabra, RM
    [J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2000, 23 (16) : 2563 - 2572
  • [2] The detection of radical scavenging compounds in crude extract of borage (Borago officinalis L.) by using an on-line HPLC-DPPH method
    Bandoniene, D
    Murkovic, M
    [J]. JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2002, 53 (1-3): : 45 - 49
  • [3] On-line HPLC-DPPH screening method for evaluation of radical scavenging phenols extracted from apples (Malus domestica L.)
    Bandoniene, D
    Murkovic, M
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (09) : 2482 - 2487
  • [4] Detection and activity evaluation of radical scavenging compounds by using DPPH free radical and on-line HPLC-DPPH methods
    Bandoniene, D
    Murkovic, M
    Pfannhauser, W
    Venskutonis, PR
    Gruzdiene, D
    [J]. EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2002, 214 (02) : 143 - 147
  • [5] Bandoniene D, 2001, NAHRUNG, V45, P105, DOI 10.1002/1521-3803(20010401)45:2&lt
  • [6] 105::AID-FOOD105&gt
  • [7] 3.0.CO
  • [8] 2-G
  • [9] Preliminary screening of antioxidant activity of some plant extracts in rapeseed oil
    Bandoniene, D
    Pukalskas, A
    Venskutonis, PR
    Gruzdiene, D
    [J]. FOOD RESEARCH INTERNATIONAL, 2000, 33 (09) : 785 - 791
  • [10] Fast separation and determination of carnosic acid and rosmarinic acid in different rosemary (Rosmarinus officinalis) extracts by capillary zone electrophoresis with ultra violet-diode array detection
    Bonoli, M
    Pelillo, M
    Lercker, G
    [J]. CHROMATOGRAPHIA, 2003, 57 (7-8) : 505 - 512
123456