A competitive chemiluminescence enzyme immunoassay for rapid and sensitive determination of enrofloxacin

被引：31
作者：
Yu, Fei [1 ]
Wu, Yongjun [1 ]
Yu, Songcheng [1 ]
Zhang, Huili [1 ,2 ]
Zhang, Hongquan [1 ]
Qu, Lingbo [2 ]
Harrington, Peter de B. [3 ]
机构：
[1] Zhengzhou Univ, Coll Publ Hlth, Zhengzhou 450001, Peoples R China
[2] Henan Univ Technol, Sch Chem & Chem Engn, Zhengzhou 450001, Peoples R China
[3] Ohio Univ, Ctr Intelligent Chem Instrumentat, Dept Chem & Biochem, Athens, OH 45701 USA
来源：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2012年 / 93卷
关键词：
Enrofloxacin; Chemiluminescence enzyme immunoassay; Alkaline phosphatase (ALP)-adamantane (AMPPD); MILK; RESIDUES; ELISA; ASSAY;
D O I：
10.1016/j.saa.2012.03.001
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
With alkaline phosphatase (ALP)-adamantane (AMPPD) system as the chemiluminescence (CL) detection system, a highly sensitive, specific and simple competitive chemiluminescence enzyme immunoassay (CLEIA) was developed for the measurement of enrofloxacin (ENR). The physicochemical parameters, such as the chemiluminescent assay mediums, the dilution buffer of ENR-McAb, the volume of dilution buffer, the monoclonal antibody concentration, the incubation time, and other relevant variables of the immunoassay have been optimized. Under the optimal conditions, the detection linear range of 350-1000 pg/mL and the detection limit of 0.24 ng/mL were provided by the proposed method. The relative standard deviations were less than 15% for both intra and inter-assay precision. This method has been successfully applied to determine ENR in spiked samples with the recovery of 103%-96%. It showed that CLEIA was a good potential method in the analysis of residues of veterinary drugs after treatment of related diseases. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：164 / 168
页数：5
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