Xanthine Oxidase Inhibitory Activities and Crystal Structures of Methoxyflavones from Kaempferia parviflora Rhizome

被引：33

作者：

Nakao, Kikuyo ^{[2
]}

Murata, Kazuya ^{[1
]}

Deguchi, Takahiro ^{[1
]}

Itoh, Kimihisa ^{[1
]}

Fujita, Takanori ^{[3
]}

Higashino, Masayuki ^{[3
]}

Yoshioka, Yuri ^{[4
]}

Matsumura, Shin-ichi ^{[4
]}

Tanaka, Rika ^{[5
]}

Shinada, Tetsuro ^{[6
]}

Ohfune, Yasufumi ^{[6
]}

Matsuda, Hideaki ^{[1
]}

机构：

[1] Kinki Univ, Fac Pharm, Osaka 5778502, Japan

[2] Kinki Univ, Res Inst Oriental Med, Osaka 5898511, Japan

[3] Japan Tablet Corp, Kyoto 6110041, Japan

[4] Inabata Koryo Co Ltd, Yodogawa Ku, Osaka 5320027, Japan

[5] Osaka City Univ, Grad Sch Engn, Osaka 5588585, Japan

[6] Osaka City Univ, Grad Sch Sci, Osaka 5588585, Japan

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2011年 / 34卷 / 07期

关键词：

Zingiberaceae; Kaempferia parviflora rhizome; xanthine oxidase; methoxyflavone; crystal structure; FLAVONOIDS; REQUIREMENTS;

D O I：

10.1248/bpb.34.1143

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Kaempferia parviflora (KP), a Zingiberaceae plant, is used as a folk medicine in Thailand for the treatment of various symptoms, including general pains, colic gastrointestinal disorders, and male impotence. In this study, the inhibitory activities of KP against xanthine oxidase (XOD) were investigated. The extract of KP rhizomes showed more potent inhibitory activity (38% at 500 mu g/ml) than those of the other Zingiberaceae plants tested. Ten methoxyflavones were isolated from the KP extract as the major chemical components and their chemical structures were elucidated by X-ray crystallography. The structurally confirmed methoxyflavones were subjected to the XOD inhibitory test. Among them, 3,5,7,4',5'-pentamethoxyflavone and 3',4',5,7-tetramethoxyflavone showed inhibitory activities (IC50 of 0.9 and >4 mM, respectively) and their modes of inhibition are clarified as competitive/non-competitive mixed type. To the best of our knowledge, this is the first report to present the inhibitory activities of KP, 3,5,7,4',5'-pentamethoxyflavone and 3',4',5,7-tetramethoxyflavone against XOD.

引用

页码：1143 / 1146

页数：4

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