Dehydroepiandrosterone, epiandrosterone and synthetic derivatives inhibit Junin virus replication in vitro

被引：21

作者：

Acosta, Eliana G. ^{[1
]}

Bruttomesso, Andrea C. ^{[2
]}

Bisceglia, Juan A. ^{[2
]}

Wachsman, Monica B. ^{[1
]}

Galagovsky, Lydia R. ^{[2
]}

Castilla, Viviana ^{[1
]}

机构：

[1] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Virol Lab, Dept Quim Biol, Buenos Aires, DF, Argentina

[2] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Organ, UMYMFOR, Buenos Aires, DF, Argentina

来源：

VIRUS RESEARCH | 2008年 / 135卷 / 02期

关键词：

arenavirus; Junin virus; steroid; dehydroepiandrosterone; DHEA; antiviral activity;

D O I：

10.1016/j.virusres.2008.03.014

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In the present paper the in vitro antiviral activity of dehydroepiandrosterone (DHEA), epiandrosterone (EA) and 16 synthetic derivatives against Junin virus (JUNV) replication in Vero cells was studied. DHEA and EA caused a selective inhibition of the replication of JUNV and other members of the Arenaviridue family such as Pichinde virus and Tacaribe virus. The compounds were not virucidal to cell-free JUNV. The impairment of viral replication was not due to an inhibitory effect of the steroids on virus adsorption or internalization. An inhibitory effect of the compounds on JUNV protein synthesis and both intracellular and extracellular virus production was demonstrated. A partial inhibitory action on cell surface expression of JUNV glycoprotein G1 was also detected on DHEA- and EA-treated cultures. Like DHEA and EA, three compounds obtained from EA by chemical synthesis showed selectivity indexes higher than ribavirin, the only antiviral compound that has shown partial efficacy against arenavirus infections. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：203 / 212

页数：10

共 31 条

[1]

BRADLEY WG, 1995, VET IMMUNOL IMMUNOP, V46, P159, DOI 10.1016/0165-2427(94)07015-Y

[2] Antiviral mode of action of a synthetic brassinosteroid against Junin virus replication [J].