Comparison of colorimetric methods for the quantification of model proteins in aqueous two-phase systems

被引：12

作者：

Glyk, Anna ^{[1
]}

Heinisch, Sandra L. ^{[1
]}

Scheper, Thomas ^{[1
]}

Beutel, Sascha ^{[1
]}

机构：

[1] Leibniz Univ Hannover, Inst Tech Chem, D-30167 Hannover, Germany

来源：

ANALYTICAL BIOCHEMISTRY | 2015年 / 477卷

关键词：

Aqueous two-phase system; Protein quantification; Colorimetric analysis; Bradford assay; BCA assay; BRADFORD METHOD; INTERFERENCE; EXTRACTION; MECHANISM;

D O I：

10.1016/j.ab.2015.02.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In the current study, the quantification of different model proteins in the presence of typical aqueous two-phase system components was investigated by using the Bradford and bicinchoninic acid (BCA) assays. Each phase-forming component above I and 5 wt% had considerable effects on the protein quantification in both assays, respectively, resulting in diminished protein recoveries/absorption values by increasing poly(ethylene glycol) (PEG)/salt concentration and PEG molecular weight. Therefore, a convenient dilution of both components (up to 1 and 5 wt%) before protein quantification is recommended in both assays, respectively, where the BCA assay is favored in comparison with the Bradford assay. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：35 / 37

页数：3

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