Genetic insights unraveling quorum quenching potential of indigenous isolates from an anaerobic membrane bioreactor

被引：20
作者：
Waheed, Hira [1 ]
Mehmood, Ch Tahir [1 ,2 ]
Li, Yiwei [1 ]
Yang, Yongyu [1 ]
Xiao, Yeyuan [1 ]
机构：
[1] Shantou Univ, Dept Civil & Environm Engn, Shantou 515063, Guangdong, Peoples R China
[2] Guangdong Technion Israel Inst Technol, Dept Chem Engn, Shantou 515063, Guangdong, Peoples R China
来源：
SCIENCE OF THE TOTAL ENVIRONMENT | 2022年 / 811卷
基金：
中国国家自然科学基金;
关键词：
Biofouling control; Anaerobic quorum quenching; Whole-genome sequencing; Micrococcus luteus; Lysinibacillus capsici; TOOL;
D O I：
10.1016/j.scitotenv.2021.152349
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Despite a few reports of quorum quenching (QQ) in anaerobic membrane bioreactors (AnMBRs), the sensing, regulation and degradation mechanism for quorum sensing (QS) signals by indigenous QQ isolates have been barely studied. This study employed isolation and screening of indigenous QQ strains from anaerobic sludge for acyl-homoserine lactones (AHLs) degradation and membrane biofouling control. High-quality whole genome sequences of Micrococcus luteus anQ-m1, Bacillus pacificus anQ-h4, and Lysinibacillus capsici anQ-h6 were obtained, with a genome size of 2.5, 5.6, and 4.7 Mbp, respectively. Amidase-encoding amiE was the only QQ gene in anQ-m1, while anQ-h6 carries both amiE and lactonase-encoding aiiB genes. Genes responsible for QS autoinducer synthesis were not identified in anQ-m1 and anQ-h6, suggesting low potential of biofilm promotion via QS. Despite a peptidic QS system responsible for biofilm formation, anQ-h4 bears the most comprehensive QQ system, including amiE-amidase, aiiA-lactonase, Science of the Total Environment 811 specialIntscript 152349 CYP102A5-cytochrome oxidoreductase , lsrK-autoinducer-2 kinase. This study elucidates QS , QQ mechanisms of potential anaerobes and provides fundamentals for designing QQ consortia to effectively control biofouling in AnMBRs.
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页数：11
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