Near-infrared and lysosome-targetable fluorescent probe based on phenoxazinium for hydrogen peroxide detection

被引：20

作者：

Chen, Yuan ^{[1
]}

Zhu, Wei-Jin ^{[1
]}

Wei, Xue-Rui ^{[2
]}

Xu, Yu-Jie ^{[2
]}

Sun, Ru ^{[1
]}

Ge, Jian-Feng ^{[1
,3
]}

机构：

[1] Soochow Univ, Coll Chem Chem Engn & Mat Sci, 199 RenAi Rd, Suzhou 215123, Peoples R China

[2] Soochow Univ, Sch Radiat Med & Protect, Collaborat Innovat Ctr Radiat Med Jiangsu Higher, State Key Lab Radiat Med & Protect, Suzhou 215123, Peoples R China

[3] Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Jiangsu Key Lab Med Opt, Suzhou 215163, Peoples R China

来源：

ANALYTICAL METHODS | 2018年 / 10卷 / 30期

基金：

中国国家自然科学基金;

关键词：

LIVING CELLS; SELECTIVE DETECTION; H2O2; VISUALIZATION; MITOCHONDRIA; PH; SIGNALS; DESIGN;

D O I：

10.1039/c8ay01403e

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new near-infrared and lysosome-targetable fluorescent probe based on phenoxazinium was designed and prepared for hydrogen peroxide detection. This probe used phenoxazinium as the fluorophore acceptor and a methyl(phenyl)sulfane unit as the recognition group through a photoinduced electron transfer mechanism. With the addition of H2O2, the methyl(phenyl)sulfane unit of the probe was oxidized to a sulfoxide unit, causing enhanced fluorescence intensity at 676 nm due to the inhibition of photoinduced electron transfer. This probe not only showed competitive sensitivity, good selectivity, and a relatively fast response time, but also selectively targeted lysosomes of HeLa cells, indicating that it could be applied to detecting and visualizing H2O2 in HeLa cell lysosomes.

引用

页码：3754 / 3758

页数：5

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