Telomerase activity in urine sediments as a tool for noninvasive detection of bladder cancer

被引：11
作者：
Dettlaff-Pokora, A
Matuszewski, M
Schlichtholz, B
机构：
[1] Med Univ Gdansk, Dept Biochem, PL-80211 Gdansk, Poland
[2] Med Univ Gdansk, Dept Urol, PL-80742 Gdansk, Poland
来源：
CANCER LETTERS | 2005年 / 222卷 / 01期
关键词：
telomerase activity; bladder cancer; urine sediments; TRAP assay;
D O I：
10.1016/j.canlet.2004.09.004
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Telomerase is extensively investigated as potential diagnostic and prognostic market in human tumors. In this study, we determined telomerase activity in histological specimens and voided urine of 52 human bladder cancers. Using the PCR-ELISA method telomerase activity was found in 2 1 (88%) of the 24 tumor tissues and in the corresponding sediments from voided urine of patients with superficial bladder carcinoma (Ta/T1). In case of muscle-invasive tumors (T2-T4), telomerase activity was found in 27 (96%) of the 28 tumor tissues and in 26 (93%) of the 28 urine sediments. Enzyme activity was not detected in 13 control urine sediments. Telomerase activity was not significantly associated with clinicopathological parameters supporting the diagnostic rather than prognostic value of this marker in bladder cancer. The present study demonstrates that telomerase activity detection in voided urine has high potential for noninvasive diagnosis of superficial bladder tumors. (c) 2004 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：83 / 88
页数：6
相关论文
共 39 条
[1]   TELOMERE LENGTH PREDICTS REPLICATIVE CAPACITY OF HUMAN FIBROBLASTS [J].
ALLSOPP, RC ;
VAZIRI, H ;
PATTERSON, C ;
GOLDSTEIN, S ;
YOUNGLAI, EV ;
FUTCHER, AB ;
GREIDER, CW ;
HARLEY, CB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (21) :10114-10118
[2]   Limitations of urinary telomerase activity measurement in urothelial cancer [J].
Arai, Y ;
Yajima, T ;
Yagihashi, A ;
Kobayashi, D ;
Kameshima, H ;
Sasaki, M ;
Tanaka, K ;
Kuwajima, K ;
Miyao, N ;
Tsukamoto, T ;
Watanabe, N .
CLINICA CHIMICA ACTA, 2000, 296 (1-2) :35-44
[3]   Comparison of human telomerase reverse transcriptase messenger RNA and telomerase activity as urine markers for diagnosis of bladder carcinoma [J].
Bialkowska-Hobrzanska, H ;
Bowles, L ;
Bukala, B ;
Joseph, MG ;
Fletcher, R ;
Razvi, H .
MOLECULAR DIAGNOSIS, 2000, 5 (04) :267-277
[4]   STRUCTURE AND FUNCTION OF TELOMERES [J].
BLACKBURN, EH .
NATURE, 1991, 350 (6319) :569-573
[5]   Extension of life-span by introduction of telomerase into normal human cells [J].
Bodnar, AG ;
Ouellette, M ;
Frolkis, M ;
Holt, SE ;
Chiu, CP ;
Morin, GB ;
Harley, CB ;
Shay, JW ;
Lichtsteiner, S ;
Wright, WE .
SCIENCE, 1998, 279 (5349) :349-352
[6]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[7]   Telomerase regulation during entry into the cell cycle in normal human T cells [J].
Buchkovich, KJ ;
Greider, CW .
MOLECULAR BIOLOGY OF THE CELL, 1996, 7 (09) :1443-1454
[8]  
FERLAY J, 2001, GLOBCAN 2000 CANC IN
[9]   A comparison of BTA stat, hemoglobin dipstick, telomerase and vysis urovysion assays for the detection of urothelial carcinoma in urine [J].
Halling, KC ;
King, W ;
Sokolova, IA ;
Karnes, RJ ;
Meyer, RG ;
Powell, EL ;
Sebo, TJ ;
Cheville, JC ;
Clayton, AC ;
Krajnik, KL ;
Ebert, TA ;
Nelson, RE ;
Burkhardt, HM ;
Ramakumar, S ;
Stewart, CS ;
Pankratz, VS ;
Lieber, MM ;
Blute, ML ;
Zincke, H ;
Seelig, SA ;
Jenkins, RB ;
O'Kane, DJ .
JOURNAL OF UROLOGY, 2002, 167 (05) :2001-2006
[10]   Telomerase activity in the regenerative basal layer of the epidermis in human skin and in immortal and carcinoma-derived skin keratinocytes [J].
HarleBachor, C ;
Boukamp, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (13) :6476-6481
1234