Electrospun scaffold containing TGF-β1 promotes human mesenchymal stem cell differentiation towards a nucleus pulposus-like phenotype under hypoxia

被引：13

作者：

Cui, Xiang ^{[1
]}

Liu, Minghan ^{[1
]}

Wang, Jiaxu ^{[1
]}

Zhou, Yue ^{[1
]}

Xiang, Qiang ^{[2
]}

机构：

[1] Third Mil Med Univ, Xinqiao Hosp, Dept Orthoped, Chongqing 400037, Peoples R China

[2] Third Mil Med Univ, Southwest Hosp, Dept Emergency, Chongqing 400038, Peoples R China

来源：

IET NANOBIOTECHNOLOGY | 2015年 / 9卷 / 02期

关键词：

MARROW STROMAL CELLS; CONTROLLED-RELEASE; IN-VITRO; INTERVERTEBRAL DISCS; STRUCTURED FIBERS; GROWTH; DEGENERATION; MODULATION; DELIVERY; TRANSPLANTATION;

D O I：

10.1049/iet-nbt.2014.0006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The study was aimed at evaluating the effect of electrospun scaffold containing TGF-beta 1 on promoting human mesenchymal stem cells (MSCs) differentiation towards a nucleus pulposus-like phenotype under hypoxia. Two kinds of nanofibrous scaffolds containing TGF-beta 1 were fabricated using uniaxial electrospinning (Group I) and coaxial electrospinning (Group II). Human MSCs were seeded on both kinds of scaffolds and cultured in a hypoxia chamber (2% O-2), and then the scaffolds were characterised. Cell proliferation and differentiation were also evaluated after 3 weeks of cell culture. Results showed that both kinds of scaffolds shared similar diameter distributions and protein release. However, Group I scaffolds were more hydrophilic than that of Group II. Both kinds of scaffolds induced the MSCs to differentiate towards the nucleus pulposus-type phenotype in vitro. In addition, the expression of nucleus pulposus-associated genes (aggrecan, type II collagen, HIF-1 alpha and Sox-9) in Group I increased more than that of Group II. These results indicate that electrospinning nanofibrous scaffolds containing TGF-beta 1 supports the differentiation of MSCs towards the pulposus-like phenotype in a hypoxia chamber, which would be a more appropriate choice for nucleus pulposus regeneration.

引用

页码：76 / 84

页数：9

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