Identification of a very long chain polyunsaturated fatty acid Δ4-desaturase from the microalga Pavlova lutheri

被引：66

作者：

Tonon, T ^{[1
]}

Harvey, D ^{[1
]}

Larson, TR ^{[1
]}

Graham, IA ^{[1
]}

机构：

[1] Univ York, Dept Biol, CNAP, York YO10 5YW, N Yorkshire, England

来源：

FEBS LETTERS | 2003年 / 553卷 / 03期

关键词：

very long chain polyunsaturated fatty acid; docosahexaenoic acid; Delta 4-d esaturase; Pavlova lutheri;

D O I：

10.1016/S0014-5793(03)01078-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pavlova lutheri, a marine microalga, is rich in the very long chain polyunsaturated fatty acids (VLCPUFAs) eicosapentaenoic (20:5n-3) and docosahexacnoic (22:6n-3) acids. Using an expressed sequence tag approach, we isolated a cDNA designated Pldes1, and encoding an amino acid sequence showing high similarity with polyunsaturated fatty acid front-end desaturases. Heterologous expression in yeast demonstrated that PIDES1 desaturated 22:5n-3 and 22:4n-6 into 22:6n-3 and 22:5n-6 respectively, and was equally active on both substrates. Thus, PIDES1 is a novel VLCPUFA Delta4-desaturase. Pldes1 expression is four-fold higher during the mid-exponential phase of growth compared to late exponential and stationary phases. (C) 2003 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：440 / 444

页数：5

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