Sequences of the second internal transcribed spacer of ribosomal DNA for three species of Trichostrongylus (Nematoda: Trichostrongylidae) from sheep in Russia

被引：8
作者：
Kuznetsov, D. N.
Kuznetsova, N. A.
机构：
[1] Russian Acad Sci, Ctr Parasitol, AN Severtsov Inst Evolut, Moscow 119049, Russia
[2] Russian Acad Sci, VA Engelgardt Inst Mol Biol, Moscow 117334, Russia
来源：
HELMINTHOLOGIA | 2007年 / 44卷 / 02期
基金：
俄罗斯基础研究基金会;
关键词：
trichostrongylus spp; ITS-2; interspecific differences; intraspecific variation; sheep; Russia;
D O I：
10.2478/s11687-007-0002-z
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
For the first time, DNA sequence data were obtained for three species of Trichostrongylus from Russia. Internal transcribed spacer (ITS-2) of ribosomal DNA was sequenced for T. axei, T. colubriformis and T. probolurus from sheep from the Moscow region. ITS-2 rDNA length was estimated as 238 nucleotides for T. colubriformis and T. probolurus and 237 nucleotides for T. axei. The G+C content of the ITS-2 sequences of T. colubriformis, T. axei and T. probolurus were 31%, 32% and 34% respectively. The level of interspecific differences in ITS-2 of rDNA of T. axei, T. probolurus and T. colubriformis ranged from 3 to 4%. The ITS-2 sequences from the Russian specimens were compared with those of T. axei, T. probolurus and T. colubriformis from Australia and Germany. Intraspecific variation ranged from 0% in T. colubriformis to 3.0% in T. axei.
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页码：43 / 46
页数：4
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