Inhibitory effect of minocycline on osteoclastogenesis in mouse bone marrow cells

被引:16
作者
Nagasawa, Tsuneyasu [1 ]
Arai, Michitsugu [1 ]
Togari, Akifumi [1 ]
机构
[1] Aichi Gakuin Univ, Sch Dent, Dept Pharmacol, Chikusa Ku, Nagoya, Aichi 4648650, Japan
关键词
Minocycline; Tetracycline; Osteoclast; Osteoclastogenesis; Bone marrow cells; RANKL; DC-STAMP; CHRONIC PERIODONTITIS; TETRACYCLINES; DIFFERENTIATION; RESORPTION; RANKL; MICROSPHERES; ANTIBIOTICS; HOMEOSTASIS; MODULATION;
D O I
10.1016/j.archoralbio.2011.02.002
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Objective: To study the effects of minocycline hydrochloride (MINO) on the formation of tartrate-resistant acid phosphatase (TRAP) staining-positive multinucleated osteoclast-like cells in mouse bone marrow cells (BMCs) treated with 1 alpha,25(OH)(2)D-3 or soluble receptor activator of nuclear factor-kappa B ligand (s-RANKL). Materials and methods: Mouse BMCs were cultured in alpha-modified minimum essential medium containing foetal calf serum (10%) and tetracyclines (2.5, 5 and 10 mu M), such as MINO, tetracycline hydrochloride (TC), oxytetracycline hydrochloride (OXT) or doxycycline (DOXY) in the presence of 1 alpha,25(OH)(2)D-3 (10 nM) or s-RANKL (20 ng/ml) for 7 days, and the number of TRAP staining-positive osteoclast-like cells was counted. In RNA isolated from BMCs treated with 1 alpha,25(OH)(2)D-3 or s-RANKL in the presence or absence of MINO, the expressions of osteoclast differentiation relating to mRNA were analysed by reverse transcription-polymerase chain reaction. Cell viability was examined in mouse BMCs and rabbit osteoclasts treated with MINO (0.25-20 mu M and 2-50 mu M, respectively) for 24 h. Results: MINO, TC, OXT or DOXY inhibited 1 alpha,25(OH)(2)D-3-induced osteoclast-like cell formation in mouse BMCs dose dependently. MINO suppressed 1 alpha,25(OH)(2)D-3-induced up-regulation of mRNA expressions of TRAP, cathepsin K, carbonic anhydrase II, and calcitonin receptor, but not RANKL. MINO inhibited s-RANKL-induced osteoclast-like cell formation and up-regulation of mRNA expressions for nuclear factor of activated T-cells c1 (NFATc1), a key regulator of osteoclast differentiation; however, MINO had no effects on the viability of mouse BMCs and rabbit osteoclasts. Conclusion: MINO inhibits RANKL-induced osteoclastogenesis via down-regulation of NFATc1 mRNA expression in osteoclast precursor cells. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:924 / 931
页数:8
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