Developmental alterations in DNA methylation during gametogenesis from primordial germ cells to sperm

被引：27

作者：

Ben Maamar, Millissia ^{[1
]}

Beck, Daniel ^{[1
]}

Nilsson, Eric ^{[1
]}

McCarrey, John R. ^{[2
]}

Skinner, Michael K. ^{[1
]}

机构：

[1] Washington State Univ, Sch Biol Sci, Ctr Reprod Biol, Pullman, WA 99164 USA

[2] Univ Texas San Antonio, Dept Biol, San Antonio, TX 78249 USA

来源：

ISCIENCE | 2022年 / 25卷 / 02期

关键词：

EPIGENETIC TRANSGENERATIONAL INHERITANCE; SPERMATOGONIAL STEM-CELLS; UNDIFFERENTIATED SPERMATOGONIA; FUNCTIONAL-ANALYSIS; MEIOTIC INITIATION; VISCERAL ENDODERM; GENE-EXPRESSION; CORD FORMATION; RETINOIC ACID; SERTOLI-CELLS;

D O I：

10.1016/j.isci.2022.103786

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Because epigenetics is a critical component for gene expression, the hypothesis was tested that DNA methylation alterations are dynamic and continually change throughout gametogenesis to generate the mature sperm. Developmental alterations and stage-specific DNA methylation during gametogenesis from primordial germ cells (PGCs) to mature sperm are investigated. Individual developmental stage germ cells were isolated and analyzed for differential DNA methylation regions (DMRs). The number of DMRs was highest in the first three comparisons with mature PGCs, prospermatogonia, and spermatogonia. The most statistically significant DMRs were present at all stages of development and had variations involving both increases or decreases in DNA methylation. DMR-associated genes were identified and correlated with gene functional categories, pathways, and cellular processes. Observations identified a dynamic cascade of epigenetic changes during development that is dramatic during the early developmental stages. Complex epigenetic alterations are required to regulate genome biology and gene expression during gametogenesis.

引用

页数：30

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