Development of fluorescence sensor and test paper based on molecularly imprinted carbon quantum dots for spiked detection of domoic acid in shellfish and lake water

Excessive levels of domoic acid (DA) in edible shellfish and water can seriously threaten human health and even cause death. Herein, we have developed a fluorescence sensor and test paper based on molecularly imprinted carbon quantum dots (B-CDs@MIPs) for DA analysis. In this research, the prepared carbon quantum dots (B-CDs) with good optical properties were used as the sensitive fluorescent signal probes, and the molecularly imprinted polymers (MIPs) instead of biological antibodies were employed as the specific recognition components because of their high stability and low cost. Due to the shielding effect of imprinted silicon layer and the influence of modified groups introduced in the MIPs preparation process, the fluorescence signal of B-CDs in MIPs is inhibited. Interestingly, the DA can cause the partial recovery of the fluorescence intensity of B-CDs@MIPs by passivating the modified groups on the surface of B-CDs in MIPs. According to this principle, the designed fluorescence sensor reveals outstanding stability, super anti-interference ability, and excellent sensitivity, with the detection limit down to 10 nM. The sensor has been successfully applied to the rapid and accurate spiked analysis of DA in edible shellfish and lake water, providing excellent recoveries. More importantly, the designed inexpensive and easy-to-operate fluorescent test paper can realize preliminary qualitative analysis of DA on site by naked eye, indicating its promising application potential in food and environmental analysis. (C) 2022 Elsevier B.V. All rights reserved.