Structure and function of a HECT domain ubiquitin-binding site

被引：100

作者：

Kim, Hyung Cheol ^{[1
]}

Steffen, Alanna M. ^{[2
]}

Oldham, Michael L. ^{[2
]}

Chen, Jue ^{[2
]}

Huibregtse, Jonm. ^{[1
]}

机构：

[1] Univ Texas Austin, Inst Cellular & Mol Biol, Austin, TX 78712 USA

[2] Purdue Univ, Howard Hughes Med Inst, Dept Biol Sci, W Lafayette, IN 47907 USA

来源：

EMBO REPORTS | 2011年 / 12卷 / 04期

基金：

美国国家卫生研究院;

关键词：

HECT; polyubiquitin chains; Rsp5; ubiquitin; COMPLEX; LIGASE; DETERMINANTS; SOFTWARE; INSIGHTS; CASCADE; RSP5; E2;

D O I：

10.1038/embor.2011.23

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Rsp5 ubiquitin ligase contains a non-covalent binding site for ubiquitin within the amino-terminal lobe (N-lobe) of the HECT domain, and the X-ray crystal structure of the HECT-ubiquitin complex has been determined. Hydrophobic patch residues of ubiquitin (L8, I44, V70) were crucial for interaction with Rsp5, and amino-acid alterations at the Rsp5-binding interface resulted in defects in polyubiquitination. Our results support a model in which the N-lobe-binding site acts to localize and orient the distal end of the ubiquitin chain to promote conjugation of the next ubiquitin molecule.

引用

页码：334 / 341

页数：8

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