A Case-Control Study of Follicular Fluid Cytokine Profiles in Women with Diminished Ovarian Reserve

被引：5

作者：

Abhari, Sina ^{[1
]}

Lu, Jingqiao ^{[2
]}

Hipp, Heather S. ^{[1
]}

Petritis, Brianne ^{[2
]}

Gerkowicz, Sabrina A. ^{[3
]}

Katler, Quinton S. ^{[1
]}

Yen, Haw-Han ^{[2
]}

Mao, Yingqing ^{[2
]}

Tang, Hao ^{[2
]}

Shang, Weirong ^{[1
]}

McKenzie, Laurie J. ^{[4
]}

Smith, Alicia K. ^{[5
]}

Huang, Ruo-Pan ^{[2
]}

Knight, Anna K. ^{[5
]}

机构：

[1] Emory Univ, Dept Gynecol & Obstet, Div Reprod Endocrinol & Infertil, Sch Med, 550 Peachtree St NE Suite 1800, Atlanta, GA 30308 USA

[2] RayBiotech Life Inc, 3607 Pkwy Lane, Peachtree Corners, GA 30092 USA

[3] IVF MD, 9960 Cent Pk Blvd, Boca Raton, FL 33428 USA

[4] Baylor Coll Med, Div Reprod Endocrinol & Infertil, Houston, TX USA

[5] Emory Univ, Dept Gynecol & Obstet, Div Res, Sch Med, Woodruff Mem Res Bldg, Atlanta, GA 30322 USA

来源：

REPRODUCTIVE SCIENCES | 2022年 / 29卷 / 09期

基金：

美国国家卫生研究院;

关键词：

Diminished ovarian reserve; Follicular fluid; Cytokines; In vitro fertilization; EPIDERMAL-GROWTH-FACTOR; MIGRATION INHIBITORY FACTOR; OOCYTE MATURATION; GRANULOSA-CELLS; EMBRYO; FERTILIZATION; HEALTH;

D O I：

10.1007/s43032-021-00757-2

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Ovarian reserve is an important determinant of a woman's reproductive potential, and women with diminished ovarian reserve (DOR) often seek in vitro fertilization (IVF). The underlying etiology of DOR is unknown, but follicular fluid cytokine concentrations likely play a role in follicular development and maturation. The present study seeks to investigate the expression of cytokines in follicular fluid (FF) of women with DOR undergoing IVF and explore correlated functional pathways. One hundred ninety-four women undergoing ovarian stimulation were recruited at the time of oocyte retrieval. Women were classified as having DOR if they met one or more of the following criteria: AMH < 1 ng/ml, FSH> 10 mIU/ml, and/or AFC < 10. Controls included women undergoing IVF for male factor, tubal factor due to tubal ligation, or planned oocyte cryopreservation (non-oncologic). The concentrations of 480 cytokines and related growth factors in follicular fluid were determined using a multiplex immunoassay. Fifty-nine cytokines had significantly different concentrations (53 higher and 6 lower) in the DOR relative to the control group after adjusting for age and body mass index (BMI) (false discovery rate; FDR< 0.1). Using the most informative 44 biomarkers as indicated by a random forest (RF) model, an area under the curve (AUC) of 0.78 was obtained. Thus, follicular microenvironment differs between women with DOR and normal ovarian reserve. The differentially expressed cytokines belong to diverse processes that are primarily involved in follicular maturation and ovulation. These changes may play an important role in treatment outcomes in women with DOR.

引用

页码：2515 / 2524

页数：10