Retinol and retinoic acid increase MMP-2 activity by different pathways in cultured Sertoli cells

被引：25

作者：

Dalmolin, Rodrigo J. S. ^{[1
]}

Zanotto-Filho, Alfeu ^{[1
]}

De Oliveira, Ramatis B. ^{[1
]}

Duarte, Roxane F. ^{[1
]}

Pasquali, Matheus A. B. ^{[1
]}

Moreira, Jose C. F. ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Sul, ICBS, Dept Bioquim, Lab 32,Ctr Estudos Estresse Oxidativo, Porto Alegre, RS, Brazil

来源：

FREE RADICAL RESEARCH | 2007年 / 41卷 / 12期

关键词：

MMP; retinol; retinoic acid; ROS; Sertoli cells; OXIDATIVE STRESS; MATRIX METALLOPROTEINASES; DNA-DAMAGE; VITAMIN-A; TISSUE INHIBITORS; PROTEIN; ERK; PHOSPHORYLATION; ANTIOXIDANT; ACTIVATION;

D O I：

10.1080/10715760701717427

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Diseases such as atherosclerosis, arthritis and cancer have been related with imbalance in ROS production and failures in regulation of the MMPs. Authors suggested a relationship between MPP activity and ROS. Our research group has demonstrated that retinol 7 mu M induced changes in Sertoli cell metabolism linking retinol treatment and oxidative stress. We verified MMP activity in Sertoli cells treated with vitamin A using gelatin zymography. We found that retinol (7 mu M) and retinoic acid (1nM) induced MMP-2 activity in Sertoli cells. Antioxiclants reversed retinol-induced but not retinoic acidinduced MMP-2 activity. Moreover, retinol but not retinoic acid increased ROS production quantified by DCFH-DA oxidation. We found that retinol and retinoic acid induced ERK1/2 phosphorylation, but only retinol-increased MMP-2 activity was inhibited by UO 126, an ERK 1/2 phosphorylation inhibitor. Our findings suggested that retinol-induced MMP-2 activity, but not retinoic acid-induced MMP-2 activity, was related to ERK1/2 phosphorylation and ROS production.

引用

页码：1338 / 1347

页数：10

共 52 条

[1] Oxidative stress activates extracellular signal-regulated kinases through Src and ras in cultured cardiac myocytes of neonatal rats [J].